Sequences from confirmation amplicon


Confirmation sequences for line 124E03 and hit At5g15970 are shown.
Line ID 124E03
Locus At5g15970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5212303
Primers used for PCR IH71 - 8474, show primer details
   
Sequence Name 91-K100855-0022-124-E03C-8409
GenBank Accession LN515096 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K100855-0022-124-E03C-8409
TTTGAAGACTTTTCATTCTTTACATAAATGTCATAATGTGTGTATAGATCGTTTACCTGT
TGCGCGGAAGCTCCAGCTGCAGCAGCAGCATCCTTGGCCTTGTCCAGCAGAACATTGCTC
TTCTCCTATATAACAACCATGGTCAACACAAAATCAATTACATAATTACGACAAACTAAA
TTAAACAAGAAAATGGAAGTTCTAGGAGTGATCAAGTTTCAACTGTTTTCAAAGAGTTAT
AACTTGAACAATCTATCAGTACTCTGTTCTAAGAGAGAAAGAGTACCTCAGCTTTGCCAG
CGGCCTGACCGGCTTGGAAGGCATTCTTGTTGGTCTCTGACATTTTTCAGATATTTTTTT
CTTGTAAAATCGTTTTTGATGTGTGTTTTGTTTGTGATGGATTGAGAAGATATGTGGGCT
TAAGGGTTTATATTTATAGGCCAAATGGAGATACTTGTGTGGTGCCACGTGTAATCTGAA
ACCACGTGGATTACGTTTAATGGTACGATCATTTTTGCCTTATG
   
Sequence Name 91-K100861-0022-124-E03C-IH71
GenBank Accession LN515097 [GenBank]
Sequencing primer gene specific primer, IH71: 5'-ACATAAGGCAAAAATGATCGTACC-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K100861-0022-124-E03C-IH71
CTCCNTTTGGACTATAAATATAAACCCTTAAGCCCACATATCTTCTCAATCCATCACAAA
CAAAACACACATCAAAAACGATTTTACAAGAAAAAAATATCTGAAAAATGTCAGAGACCA
ACAAGAATGCCTTCCAAGCCGGTCAGGCCGCTGGCAAAGCTGAGGTACTCTTTCTCTCTT
AGAACAGAGTACTGATAGATTGTTCAAGTTATAACTCTTTGAAAACAGTTGAAACTTGAT
CACTCCTAGAACTTCCATTTTCTTGTTTAATTTAGTTTGTCGTAATTATGTAATTGATTT
TGTGTTGACCATGGTTGTTATATAGGAGAAGAGCAATGTTCTGCTGGACAAGGCCAAGGA
TGCTGCTGCTGCAGCTGGAGCTTCCGCGCAACAGGTAAACGATCTATACACACATTATGA
CATTTATGTAAAGAATGAAAAGTCTTCAAATGACATTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
Primers used for PCR 14lf - 8474, show primer details
   
Sequence Name 69-K113920-0022-124-E03C-8409
GenBank Accession LN512250 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K113920-0022-124-E03C-8409
TTTATGTCATTTGAGACTTTTCATTCTTTACATAAATGTCATAATGTGTGTATAGATCGT
TTACCTGTTGCGCGGAAGCTCCAGCTGCAGCAGCAGCATCCTTGGCCTTGTCCAGCAGAA
CATTGCTCTTCTCCTATATAACAACCATGGTCAACACAAAATCAATTACATAATTACGAC
AAACTAAATTAAACAAGAAAATGGAAGTTCTAGGAGTGATCAAGTTTCAACTGTTTTCAA
AGAGTTATAACTTGAACAATCTATCAGTACTCTGTTCTAAGAGAGAAAGAGTACCTCAGC
TTTGCCAGCGGCCTGACCGGCTTGGAAGGCATTCTTGTTGGTCTCTGACATTTTTCAGAT
ATTTTTTTCTTGTAAAATCGTTTTTGATGTGTGTTTTGTTTGTGATGGATTGAGAAGATA
TGTGGGCTTAAGGGTTTATATTTATAGGCCAAATGGAGATACTTGTGTGGTGCCACGTGT
AATCTGAAACCACGTGGATTACGTTTAATGGTACGATCATTTTTGCCTTATGTCGGTAGC
TTTTTATTGGCTGATCAAGACATCACGTGTCGTTTTAGTTTAGTTTATCAATTTGTTATA
GAGGATATGAGA
   
Sequence Name 59-K113919-0022-124-E03C-14lf
GenBank Accession LN510056 [GenBank]
Sequencing primer gene specific primer, 14lf: 5'-GTCTCATATCCTCTATAACAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K113919-0022-124-E03C-14lf
CCAATAAAAGCTACCGACATAAGGCAAAAATGATCGTACCATTAAACGTAATCCACGTGG
TTTCAGATTACACGTGGCACCACACAAGTATCTCCATTTGGCCTATAAATATAAACCCTT
AAGCCCACATATCTTCTCAATCCATCACAAACAAAACACACATCAAAAACGATTTTACAA
GAAAAAAATATCTGAAAAATGTCAGAGACCAACAAGAATGCCTTCCAAGCCGGTCAGGCC
GCTGGCAAAGCTGAGGTACTCTTTCTCTCTTAGAACAGAGTACTGATAGATTGTTCAAGT
TATAACTCTTTGAAAACAGTTGAAACTTGATCACTCCTAGAACTTCCATTTTCTTGTTTA
ATTTAGTTTGTCGTAATTATGTAATTGATTTTGTGTTGACCATGGTTGTTATATAGGAGA
AGAGCAATGTTCTGCTGGACAAGGCCAAGGATGCTGCTGCTGCAGCTGGAGCTTCCGCGC
AACAGGTAAACGATCTATACACACATTATGACATTTATGTAAAGAATGAAAAGTCTTCAA
ATGACATTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCA

Last Updated on 06.12.2018 16:11