Sequences from confirmation amplicon


Confirmation sequences for line 124F01 and hit At1g74320 are shown.
Line ID 124F01
Locus At1g74320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27941449
Primers used for PCR ML37 - 8474, show primer details
   
Sequence Name 63-K100815-0022-124-F01C-8409
GenBank Accession LN510931 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K100815-0022-124-F01C-8409
AGCCATTTTTTTCTGTCCGGATGAGATCAGGACATTTGAGTTCATGTCGAAGCATGGTCA
TGGACCTTTGTTGTTAGGTCGGTTTGGTAATGGTCGTATCGAAGAGTTCCTCCATGCACG
GGTAATTATTTTTTTAATATCTGATTCTGTGTGTTCCTAAGTTATTGAGTATCTTTGCAC
TTCCCTGGTTTTTTTCATTCTTCAGGTAATTCTTGTGAATCTTTTTGTATTTGTTTACTG
AATTTGTAACTTGTTTGCTGAACTATGAACTTTCAGACCCTATCTGCTTGTGACTTGCGT
GATCCTGAAATATCTGGTCGAATAGCAACTAGGATGAAGGAGTTTCATGGTCTTGAAATG
CCTGGTGCGAAAAAGGCTTTGCTTTGGGACA
   
Sequence Name 63-K100827-0022-124-F01C-ML37
GenBank Accession LN510933 [GenBank]
Sequencing primer gene specific primer, ML37: 5'-AATGTACCGTAATCTGTCCCAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K100827-0022-124-F01C-ML37
CATTTCAGACCATGAAACTCCTTCATCCTAGTTGCTATTCGACCAGATATTTCAGGATCA
CGCAAGTCACAAGCAGATAGGGTCTGAAAGTTCATAGTTCAGCAAACAAGTTACAAATTC
AGTAAACAAATACAAAAAGATTCACAAGAATTACCTGAAGAATGAAAAAAACCAGGGAAG
TGCAAAGATACTCAATAACTTAGGAACACACAGAATCAGATATTAAAAAAATAATTACCC
GTGCATGGAGGAACTCTTCGATACGACCATTACCAAACCGACCTAACAACAAAGGTCCAT
GACCATGCTTCGACATGAACTCAAATGTCCTGATCTCATCCGGACAGAAAAAAATGGCTC
TNAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG

Last Updated on 06.12.2018 16:11