Sequences from confirmation amplicon


Confirmation sequences for line 125A02 and hit F15B8 are shown.
Line ID 125A02
Locus F15B8 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21333980
Primers used for PCR MZ15 - 8474, show primer details
   
Sequence Name 19-K017255-022-125-A02C-MZ15
GenBank Accession LN494549 [GenBank]
Sequencing primer gene specific primer, MZ15: 5'-AAATGGGTACCTTCAAGGAAGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K017255-022-125-A02C-MZ15
GTATGCTAAACGTGTAATGCTCAGCCTAGTGTTCACATAATCCAGCAAAGACTAGAAGAA
CTCAAGAAAACTGGACTTTTATCTCAATCCTATTCTTCTAGTTCTTCCTCCACCGAATCA
AAAACTAATACTAGCTTTCTTGATGAGAAGACCAGCAAGGGAGAAACAGACAATATGTTC
GAAGGTGGTGATCAGAAGAAACCAGAGATCGACCTGACCGAGTTTCTTCAGCAACTAGGA
ATCTTGAAGGATGAAAATGAAGCAGAACCAAGTGAGGTAGCAGAGTGTCATTCCCCTCCA
CCATGGAACGAGCAAGAAGAAACTGGAAGTCCTTTCAGAACTGAGAATTTCAGCTGGGAT
ACCCTGATCGAGATGCCAAGAAGTGAAACCACAACTATGCAATTTGACTCCAGCAACTTC
GGAAGCTATGATTTTGAGGATGATGTATCCTTCCCTTCCATCTGGGACTACTACGGAAGC
TTAGATTGAGTAAAAGCAATTTAAGGTAGATCAAGATTCAGAAGTACACAAATGGTTTTG
GATTTAGTGTAGCGTTTTGGAAAAGAGACATAGGTAGTGAGAGTGCAGTCTTTTATTATG
CAGCAATAAAGTGAGTCAGTGTACAACCGAGTTGTTCGCTTTTTTTGGTATATTAATGAA
GCATGTTCATTTTTTCGCTATCCTTTAGCTCGATAGTTTAAATAGTAAGTGAAAGTCACG
GCCCTAATAGAATGATATGTT
   
Sequence Name 20-K017255-022-125-A02C-8409
GenBank Accession LN495001 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K017255-022-125-A02C-8409
GACATGAAGCCATTTACAACTGAATATATCCTGAGTCCCCGGAAAATGGCTATTAGGGCG
CTGAAATCTCAACCGGTTTGTCTCCATGAAAGGGGATATGATAGGTAAGCTTTTTTGCAT
TGTTACTTGCACAAAACATATCATTCTATTAGGGCCGTGACTTTCACTTACTATTTAAAC
TATCGAGCTAAAGGATAGCGAAAAAATGAACATGCTTCATTAATATACCAAAAAAAGCGA
ACAACTCGGTTGTACACTGACTCACTTTATTGCTGCATAATAAAAGACTGCACTCTCACT
ACCTATGTCTCTTTTCCAAAACGCTACACTAAATCCAAAACCATTTGTGTACTTCTGAAT
CTTGATCTACCTTAAATTGCTTTTACTCAATCTAAGCTTCCGTAGTAGTCCCAGATGGAA
GGGAAGGATACATCATCCTCAAAATCATAGCTTCCGAAGTTGCTGGAGTCAAATTGCATA
GTTGTGGTTTCACTTCTTGGCATCTCGATCAGGGTATCCCAGCTGAAATTCTCAGTTCTG
AAAGGACTTCCAGTTTCTTCTTGCTCGTTCCATGGTGGAGGGGAATGACACTCTGCTACC
TCACTTGGTTCTGCTTCATTTTCATCCTTCAAGATTCCTAGTTGCTGAAAAAACTCGGTC
AGGTCGATCTCTGGTTTCTTCTGATCACCACCTTCGAACATATTGTCTGTTTCTCCCTTG
CTGGTCTTCTCATCAAGAAAGCTAGTATTAGTTTTTG

Last Updated on 06.12.2018 16:11