Sequences from confirmation amplicon


Confirmation sequences for line 125D02 and hit At3g23600 are shown.
Line ID 125D02
Locus At3g23600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8473862
Primers used for PCR AC68 - 8474, show primer details
   
Sequence Name 54-K104214-0022-125-D02C-AC68
GenBank Accession LN508466 [GenBank]
Sequencing primer gene specific primer, AC68: 5'-ATCAGGAACCACAACATAGAATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K104214-0022-125-D02C-AC68
TCGCAGCGCCCTTAGACATAAAATTAAGACTTAACACTCCATAAACATTCCTCCCTATTT
GTACAAGATAAACTTGAGATAATGCAATGCAATCAAAGCAATGATTCAAATTAAACATTT
AGATCTTATCAAATGAGTTCAGAAAAGACAAGTCAACTTAAAGACACATGATAGGTATGC
TTCATCTTGTACATAATCCAAAACCAACAAAACTATTTGCAGGCTCAAATGAAAATTCTA
ACTAGACCTCATATGTGGCTACTAAATACTATCTAATTCCTTCAAAGAAACAAACCCAAG
TTAAAGATTCCATTTTTAATGGTTGAATCGATGAAGAGGGAAGAATTAGTAGTAGTACCT
TAAGTTTGGAGCTTCAAAACCTGAAGAACAAAAACGTACCTAAAGTCAGAGTCAACAATG
ATGCAATGATCAAATCAACAAAACGGATCCAAAAAGAGAAAAACAAAAACCCTAATTACT
CCGATCATATTTCAACTAAAACAAAGAAAGTCAAAACAGATCAAAGCAAAGAAAGAAGAG
TATTTTACCAAAAATATCAGAAATGAGGAGGACGCAGAGCTTGGACTCGGCAGAGCCGGA
GACGTAGGCATCAAGCCCACCCAGCTTCTCGACATGACCCGACCCGGAAACCGGGTTAAG
AGTTGGCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGGTCATATGGAGAAAAAGAAAGAGTAATTTACCATTTTTTTTCAATTCAAA
AATGTAGATGT
   
Sequence Name 14-K104215-0022-125-D02C-8409
GenBank Accession LN492489 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K104215-0022-125-D02C-8409
GCCATTTACAATTGAATATGCCAACTCTTAACCCGGTTTCCGGGTCGGGTCATGTCGAGA
AGCTGGGTGGGCTTGATGCCTACGTCTCCGGCTCTGCCGAGTCCAAGCTCTGCGTCCTCC
TCATTTCTGATATTTTTGGTAAAATACTCTTCTTTCTTTGCTTTGATCTGTTTTGACTTT
CTTTGTTTTAGTTGAAATATGATCGGAGTAATTAGGGTTTTTGTTTTTCTCTTTTTGGAT
CCGTTTTGTTGATTTGATCATTGCATCATTGTTGACTCTGACTTTAGGTACGTTTTTGTT
CTTCAGGTTTTGAAGCTCCAAACTTAAGGTACTACTACTAATTCTTCCCTCTTCATCGAT
TCAACCATTAAAAATGGAATCTTTAACTTGGGTTTGTTTCTTTGAAGGAATTAGATAGTA
TTTAGTAGCCACATATGAGGTCTAGTTAGAATTTTCATTTGAGCCTGCAAATAGTTTTGT
TGGTTTTGGATTATGTACAAGATGAAGCATACCTATCATGTGTCTTTAAGTTGACTTGTC
TTTTCTGAACTCATTTGATAAGATCTAAATGTTTAATTTGAATCATTGCTTTGATTGCAT
TGCATTATCTCAAGTTTATCTTGTACAACTATGGAGGAATGTTTATGGAGTGTTAAGTCT
TAATTTTATGTCTTAAGGGCGCTTGCGGACAAGGTTGCAGCTTCTGGATTCTATGT

Last Updated on 06.12.2018 16:11