Sequences from confirmation amplicon


Confirmation sequences for line 125D10 and hit At2g24740 are shown.
Line ID 125D10
Locus At2g24740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10531298
Primers used for PCR IN38 - 3144, show primer details
   
Sequence Name 34-K033707-022-125-D10C-IN38
GenBank Accession LN501318 [GenBank]
Sequencing primer gene specific primer, IN38: 5'-ACTAGTCCAATAGACCGAGAAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K033707-022-125-D10C-IN38
ACATCAGCACCTCCTCATGTAATGGTCTCACCACTACAAAACAGAAGACCATTTGATCAA
TTCAACAACCAACCTTATGATGCGAGTGCTGGTCCTAGTACTGGTCCAGGAAAGCGAGGA
CGTGGCCGACCAAAGGGTTCGAAAAATGGGTCGAGAAAGCCAAAAAAGCCAAAAGCATAT
GACAACAACTCTACTGATGCGAGTGCTGGTCCTAGTTCTGGTCTAGGAAAACGAAGATGT
GGCCGACCAAAGAAAAAGAAAGCAATTACCAATGGCTTCATGTCCGGGAAACGACAATCT
GATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTC
TTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATC
CACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGG
GGGTCATTTCTTTGG
   
Sequence Name 34-K033707-022-125-D10C-3144
GenBank Accession LN501317 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K033707-022-125-D10C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGGACATGAAGCCATTGGTAATTGCTTTCT
TTTTCTTTGGTCGGCCACATCTTCGTTTTCCTAGACCAGAACTAGGACCAGCACTCGCAT
CAGTAGAGTTGTTGTCATATGCTTTTGGCTTTTTTGGCTTTCTCGACCCATTTTTCGAAC
CCTTTGGTCGGCCACGTCCTCGCTTTCCTGGACCAGTACTAGGACCAGCACTCGCATCAT
AAGGTTGGTTGTTGAATTGATCAAATGGTCTTCTGTTTTGTAGTGGTGAGACCATTACAT
GAGGAGGTGCTGATGTTGCGTGGGCGTCTTCATTGACTTCATGAGATGCTTCTCGGTCTA
TTGGACTAG

Last Updated on 06.12.2018 16:11