Sequences from confirmation amplicon


Confirmation sequences for line 125H11 and hit At4g00260 are shown.
Line ID 125H11
Locus At4g00260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:116730
Primers used for PCR ME48 - 8474, show primer details
   
Sequence Name 16-K027636-022-125-H11C-ME48
GenBank Accession LN493291 [GenBank]
Sequencing primer gene specific primer, ME48: 5'-ACTCAAGTATCCACAAGAGCCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K027636-022-125-H11C-ME48
AGTCTAGGGTTAAAATGTGCTTTAAGGCATGCTTGTGTGTGTGCCTAAGAGTGTAGTGAA
GTAGTATAAGTAAGAGTGTGTGTGTGTCTTGTAATATTATCCAAGATATTAACAATGTAG
AAGATTAAAAGAAAAATGGTTTTTGTTCTTACTCTCTAAGACAATGAAACCTTAAGAGTT
ATATCTTACCAAATAAAAGAGTCAAACATTGATTCATTTCAATATAGAGTTTCATTTGAT
TTCTAGCTTCACCTTGGAGCAGAACTTAAGGACGCAACTTGTTTCTTCTTCCCAAATCAG
TTCCAACATGATGGATTCGTTTGCCTTCACATCGTTAGCTTGGATGAATCCTTGCCATCC
TCCTACCATTCGTAGTCTTTTTCTTTTGTCCCGCTTGCGACTCGAACCGCAGAGTCGTTA
ACCACTTCACACCATGTTTATCCAACAGAGTCATTTTAGTTTCTTCATTGATACCATTCA
TCCTCGTGAAAGGAACCGGAAGACGCTGAAACATAGATTCACACAAAAGATTCTCATTCT
ATAGACTGTTAAAAGACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACA
AATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAAT
CTNTATNTCGACGTGTCT
   
Sequence Name 15-K027636-022-125-H11C-8409
GenBank Accession LN492823 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K027636-022-125-H11C-8409
GCCATTTACAATTGTCTTTTACAGTCTATAGAATGAGAATCTTTTGTGTGAATCTATGTT
TCAGCGTCTTCCGGTTCCTTTCACGAGGATGAATGGTATCAATGAAGAAACTAAAATGAC
TCTGTTGGATAAACATGGTGTGAAGTGGTTAACGACTCTGCGGTTCGAGTCGCAAGCGGG
ACAAAAGAAAAAGACTACGAATGGTAGGAGGATGGCAAGGATTCATCCAAGCTAACGATG
TGAAGGCAAACGAATCCATCATGTTGGAACTGATTTGGGAAGAAGAAACAAGTTGCGTCC
TTAAGTTCTGCTCCAAGGTGAAGCTAGAAATCAAATGAAACTCTATATTGAAATGAATCA
ATGTTTGACTCTTTTATTTGGTAAGATATAACTCTTAAGGTTTCATTGTCTTAGAGAGTA
AGAACAAAAACCATTTTTCTTTTAATCTTCTACATTGTTAATATCTTGGATAATATTACA
AGACACACACACACTCTTACTTATACTACTTCACTACACTCTTAGGCACACACACAAGCA
TGCCTTAAAGCACATTATTAACCCTAGACTCTAGACTCTTGCACAAGCCACTCTACTACA
TTGGCT

Last Updated on 06.12.2018 16:11