Sequences from confirmation amplicon


Confirmation sequences for line 126B11 and hit At4g17550 are shown.
Line ID 126B11
Locus At4g17550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9778756
Primers used for PCR 17kb - 8474, show primer details
   
Sequence Name 64-K113393-0022-126-B11C-8409
GenBank Accession LN511243 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K113393-0022-126-B11C-8409
TGATCCGGGATCATACAAGCTTGCAAAAGTCCAACACTTCTTTTATTCTCATACCCTGAA
CCTGACCCTGATGATCCCTCCCCATCCCCTTCAACATCTGTCCCTAAATCTTCTTCTACT
TCCTCTTCTTCCTCTTCAACATCTCTCTTCCTCTTAATAAACTTCCCTGAATTAGAATTG
ATATCTGGAAACCCAACATCCTCAGGATACGCAGCCAAGAACAAATAAACAAGAACTCCT
CCTAAAGACATGACAAAACCAGGAGCTATAAAAGACCATCCCCAACCATATTCAAGAACA
CCAGCAGCAATTAAAGAACCACAAATGTTCCCAACAGAAGTATGAGCATTCCAA
   
Sequence Name 83-K113394-0022-126-B11C-17kb
GenBank Accession LN514335 [GenBank]
Sequencing primer gene specific primer, 17kb: 5'-TTTGGAATGCTCATACTTCTGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K113394-0022-126-B11C-17kb
CTTGATATGGTTGGGGATGGTCTTTTATAGCTCCTGGTTTTGTCATGTCTTTAGGAGGAG
TTCTTGTTTATTTGTTCTTGGCTGCGTATCCTGAGGATGTTGGGTTTCCAGATATCAATT
CTAATTCAGGGAAGTTTATTAAGAGGAAGAGAGATGTTGAAGAGGAAGAAGAGGAAGTAG
AAGAAGATTTAGGGACAGATGTTGAAGGGGATGGGGAGGGATCATCAGGGTCAGGTTCAG
GGTATGAGAATAAAAGAAGTGTTGGACTTTTGCAAGCTTGTATGATCCCTGGATCAGCAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTA

Last Updated on 06.12.2018 16:11