Sequences from confirmation amplicon


Confirmation sequences for line 126F10 and hit At5g45400 are shown.
Line ID 126F10
Locus At5g45400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18401299
Primers used for PCR 21ra - 8474, show primer details
   
Sequence Name 80-K115895-0022-126-F10C-21ra
GenBank Accession LN513986 [GenBank]
Sequencing primer gene specific primer, 21ra: 5'-ATTTCTGCTATACGGCTTGTCCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K115895-0022-126-F10C-21ra
AATAACGGAGATGGGACATGGCGTTGTGAGAAGTGTGACAAATGCGTGGATGAATGTGAC
TACAGGTATATATTACAGATCCAGTTACAGGACCATACAGACCTAACCTGGGCAACTGCA
TTCCAAGAAGCTGGTGAAGAGATAATGGGAATGTCTGCAAAAGATTTGTATTATGTGAAG
TATGAAAATCAGGATGAGGAGAAATTTGAAGATATCATCCGCAGTGTCGCTTTTACTAAG
TACATTTTCAAGCTGAAGATTAAAGAAGAAACTTACAGCGATGAGCAGCGGGTTAAAGCA
ACAGTTGTGAAAGCTGAAAAGCTGAATTATTCTTCAAATACCAGGTTCATGCTAGAGGCA
ATAGATAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
   
Sequence Name 50-K115894-0022-126-F10C-8409
GenBank Accession LN507281 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K115894-0022-126-F10C-8409
CATTTATCTATTGCCTCTGCATGAACCTGGTATTTGAAGAAAATTCAGCTTTTCAGCTTT
CACAACTGTTGCTTTAACCCGCTGCTCATCGCTGTAAGTTTCTTCTTTAATCTTCAGCTT
GAAAATGTACTTAGTAAAAGCGACACTGCGGATGATATCTTCAAATTTCTCCTCATCCTG
ATTTTCATACTTCACATAATACAAATCTTTTGCAGACATTCCCATTATCTCTTCACCAGC
TTCTTGGAATGCAGTTGCCCAGGTTAGGTCTGTATGGTCCTGTAACTGGATCTGTAATAT
ATACCTGTAGTCACATTCATCCACGCATTTGTCACACTTCTCACAACGCCATGTCCCATC
TCCGTTATTTGTGACCTTTTTGCTGCATGGACGATCTCCATTCATGATAGGACAAGCCGT
AAGNCAGAAATAACGGAGATGGGACATGGCGTTGTGAGAAGTGTGACAAATGCGTGGATG
AATATGACTACAGGTATATATTACAGATCCAGTTACAGGACCATACAGACCTAACCTGGG
CAACTGCATTCCAAGAAGCTGGTGAAGAGATAATGGGAATGTCTGCAAAAGATTTGTATT
ATGTGAAGTATGAAAATCAGGATGAGGAGAAATTTGAAGATATCATCCGCAGTGTCGCTT
TTACTAAGTACATTTTCAAGCTGAAGATTAAAGAAGAAACTTACAGCGATGAGCAGCGGG
TTAAAGCAACAGTTGTGAAAGCTGAAAAGCTGAATTATTCTTCAAATACNAGGTTCATGC
TAGAGGCAATAGATAAATGGCTTCATGTCCGGGAAATCTACATGGATTCGCAATGAG

Last Updated on 06.12.2018 16:11