Sequences from confirmation amplicon


Confirmation sequences for line 126H03 and hit At1g15100 are shown.
Line ID 126H03
Locus At1g15100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5194159
Primers used for PCR ML34 - 8474, show primer details
   
Sequence Name 10-K100818-0022-126-H03C-8409
GenBank Accession LN490570 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K100818-0022-126-H03C-8409
CCATTTACAATTGTATATTTGTAGGTTTCACTAGCATTTGTAGCCCCATCTTTCTTCTTC
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTCTCTCTGTTTTCTATATATATAGCTG
TCTTTGCTTAACCTATACACATTATTGTGTTCCTTTACTTTCACCTTTTATTAATTTCAT
TACTAAACTGTCAATTAGACTTTTAATTTTGGTTTATTACTAAAGGAAATTAATTATTTA
AGATACTTTTCCATACAATTTAATTTAAAATATATAAAGTGGATGTATAGTTGATTTTTT
ATTTTATTTCAAGCTATATTATGGTACGAAATCATTATTATGTTGCATATATTAATCGAT
TTGGTATATTTTTTTACAAAATACAGAAGAATACAAAGATTTTTATTATTATTTTGACCA
CCTATTTTTGTCAATTTTTATTACCAGTCTGACGATTATGTAATTTAGATCAAATGAAGT
TAGTAGTAATAAATACCAAATCTTTTTATACACGTGATCTGGTATTTTACAGATCTGATT
TTATATTACAAACCAAACGTCACTTATTTCTCTCTCTTTTTTAATTCAGACCCCTCCATT
TTGCCGTCTATGCCCACAATTCCCCGTTAGTTTTCACT
   
Sequence Name 10-K100830-0022-126-H03C-ML34
GenBank Accession LN490572 [GenBank]
Sequencing primer gene specific primer, ML34: 5'-ACACTGAAAGTGAAAACTAACGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K100830-0022-126-H03C-ML34
GGTCTGAATTAAAAGAGAGAGAAATAAGTGACGTTTGGTTTGTAATATAAAATCAGATCT
GTAAAATACCAGATCACGTGTATAAAAAGATTTGGTATTTATTACTACTAACTTCATTTG
ATCTAAATTACATAATCGTCAGACTGGTAATAAAAATTGACAAAAATAGGTGGTCAAAAT
AATAATAAAAATCTTTGTATTCTTCTGTATTTTGTAAAAAAATATACCAAATCGATTAAT
ATATGCAACATAATAATGATTTCGTACCATAATATAGCTTGAAATAAAATAAAAAATCAA
CTATACATCCACTTTATATATTTTAAATTAAATTGTATGGAAAAGTATCTTAAATAATTA
ATTTCCTTTAGTAATAAACCAAAATTAAAAGTCTAATTGACAGTTTAGTAATGAAATTAA
TAAAAGGTGAAAGTAAAGGAACACAATAATGTGTATAGGTTAAGCAAAGACAGCTATATA
TATAGAAAACAGAGAGAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAA
GATGGGGCTACAAATGCTAGTGGAAACCTACAAATATACAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATTC
Primers used for PCR ML35 - 3144, show primer details
   
Sequence Name 18-K100831-0022-126-H03C-ML35
GenBank Accession LN494221 [GenBank]
Sequencing primer gene specific primer, ML35: 5'-GGAAACCAAAGCAGATCTACAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K100831-0022-126-H03C-ML35
AGCCTCCTTCCAACACTTCTTGTGGAACCGTGTCGACATTCCAGCTTCCTCACCTCTTCA
CCTTCCTTTAACTTCGACAAACACACAACACAATCGGAGCCGCCACCACCTCCGTCACCG
CACCGGTACGAGAATAACCGATTCAAACTAAGCTGATCGGCGAGAACGATTATGTTGGCT
AGTCCCGATGCTATAGAGACATCGTCTACGGGGAGATTAGGATTTGATTTAGAGGTGAGA
CGGAGGAGGAAAGATCGGAGATGGTTGATGAAGACGGCGAGGAGAGAGAGGAGCATAAGA
GGGATTGAATCGGAAGAGACGTCATTCCCAGATGGGGATAGTGGAAGGGTCTTGCGAAGG
CGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTT
ATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGG
AGATATCA

Last Updated on 06.12.2018 16:11