Sequences from confirmation amplicon


Confirmation sequences for line 126H05 and hit At1g67980 are shown.
Line ID 126H05
Locus At1g67980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25488501
Primers used for PCR LD16 - 8474, show primer details
   
Sequence Name 41-K030459-022-126-H05C-8409
GenBank Accession LN503962 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K030459-022-126-H05C-8409
CTTTCTTTAATCAACGTCTATCGCGGTAATCTACACCTAAAGAGCATCCATTTCGATAAC
TATGTTTCTTAGTTAAAACAAACAAATAATTTTCGTAACCCCAACAATATGATTATATAT
AACAAGAAGCATTACACGGCCATCTTCAGGTAAAGCAAGAGCTGTAGTGAGAAGCGAGTA
ACCGGTGAAAACACCGATCTCGATAGTGTTTTTCGCATTCATGATCTTTACAAGCATCGA
TAGGAAATGACCCTCATCAACCGGAACCTCCATCTCGCTTCTGCGTTTTTATTTTTAAAT
TCTACAAAATTAAGCAACTATATAGCTATCTCTAAAATAATCACAAACTATTGGCAAAAT
TTTGTTTATTCCACTTCTTAAACTATATAAAGATAAAATTTCTTAATTTAAAAAAGTTGT
TATATAGTCCTATAGATTTTGTTGCACCTAAAAAAGAAAGTCATATATTAAGACTTACAA
ATTTCCGTATTTTTGGA
   
Sequence Name 42-K030459-022-126-H05C-LD16
GenBank Accession LN504346 [GenBank]
Sequencing primer gene specific primer, LD16: 5'-TTCGAAAAGCTACTGTCCAAAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K030459-022-126-H05C-LD16
AACAAAATTTTCGCCAATAGTTCGTCGAATTATTTTAGAGATAGCTATATAGTCGCTTAA
TTTTGTAGAATTTAAAAATAAAAACGCAGAAGCGAGATCGGAGGTTCCGGTTGATGAGGG
TCATTTCCTATCGATGCTTGTAAAGATCATGAATGCGAAAAACACTATCGAGATCGGTGT
TTTCACCGGTTACTCGCTTCTCACTACAGCTCTTGCTTTACCTGAAGATGGCCGTGTAAT
GCTTCTTGTTATATATAATCATATTGTTGGGGTTACAAAATTATTTGTTTGTTTTAACTA
AGAAACATAGTTATCAAAATGGATGCTCTTTGGATGTAGATTACCGCGATAGACATTGAT
AAAGAAGCTTACGAAGTTGGACTAGAGTTTATTAAGAAGGCTGGTGTGATCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
ANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGC
GAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA
Primers used for PCR 60wr - 3144, show primer details
   
Sequence Name 80-K118835-0022-126-H05C-60wr
GenBank Accession LN514000 [GenBank]
Sequencing primer gene specific primer, 60wr: 5'-AAAACTTTGGTCATATCATTTGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K118835-0022-126-H05C-60wr
AACACTANTGTGCATATGCATGTAAAATTGAAACACACATATTAGATGTCCCCAAAAAGC
TGAAAGGTGTTTATTTAATCTTTACACAANCTCTTTTTAATAGTTTACAAACACTTAAAA
AGAAAAAAAACAGTAATAATAATGTGTCGACCAGAAAAAAAAAAATTATAATGTTACAAC
AATTATTATTAAAAGTAATTTAGTGTACTAATAGTTTTGTTGCCAGACAGAACAACATCG
ACGAAACCACAACATAGAAAGAAGATAAAGAGTGAGCATCAATCAAACACACCATTACAA
GCAAAGTTGTGACAAAAGACGAAGTCGTTATTGAACCATCTAACACTTTTATTCCAAACT
CTCCGACTTTGGTTCTGCTACGTACTCTTCAAGTAGTCTTTTGATCAAACAAGACGTCTA
CAGAGCGTGATACCATCTCCAATGGAAATCTGAGAGACCTCGACTCGGGGATCCAAAGCC
AATTTCTTATTGAATTCTATAAGAGCTGCTCTATATTCTCTCATATGCTCCGGAACTCCA
TCTTCATCCTCCGCCACAAAACCAAACCACAAGGTGTTGTCGAACGCAATGATTCCCCCA
ACCTTCACCAATTTTAGAAGCCTCTCATGGAAGTTGACGTAGCTTGATTTGTCAGCATCC
GCAAATGCAAAATCAAACTCACATTTCTGCAATGTTTTTTAATGTATGACCATTAGCTAA
AAAAATATAGTTAATTAAGCTTAAGGTTAATAATAATATAAGAACACTTATTTTCACGTA
CGTCGTTCACCAATTGGTCTAAGGCCTTAAGACCATCGGAATGGATAAAATTAA
   
Sequence Name 56-K118834-0022-126-H05C-35St
GenBank Accession LN509250 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K118834-0022-126-H05C-35St
CGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGG
ATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGGGTATCGGTTTAAAGATTAAT
TTTATCCATTCCGATGGTCTTAAGGCCTTAGACCAATTGGTGAACGACGTACGTGAAAAT
ATGTGTTCTTATATTATTATTAACTTTAAGCTTAATTAACTATATTTTTTTAGCTAATGG
TCATACATTAAAAAACATTGCAGAAATGTGAGTTTGATTTTGCATTTGCGGATGCTGACA
AATCAAGCTACGTCAACTTCCATGAGAGGCTTCTAAAATTGGTGAAGGTTGGGGGAATCA
TTGCGTTCGACAACACCTTGTGGTTTGGTTTTGTGGCGGAGGATGAAGATGGAGTTCCGG
AGCATATGAGAGAATATAGAGCAGCTCTTATAGAATTCAATAAGAAATTGGCTTTGGATC
CCCGAGTCGAGGTCTCTCAGATTTCCATTGGAGATGGTATCACGCTCTGTAGACGTCTTG
TTTGATCAAAAGACTACTTGAAGAGTACGTAGCAGAACCAAAGTCGGAGAGTTTGGAATA
AAAGTGTTAGATGGTTCAATAACGACTTCGTCTTTTGTCACAACTTTGCTTGTAATGGTG
TGTTTGATTGATGCTCACTCTTTATCTTCTTTCTATGTTGTGGTTTCGTCGATGTTGTTC
TGTCTGGCAACAAAACTATTAGTACACTAAATTACTTTTAATAATAATTGTTGTAACATT
ATAATTTTTTTTTTTCTGGGCGACACATTATTATAACGGTTTTT

Last Updated on 06.12.2018 16:11