Sequences from confirmation amplicon


Confirmation sequences for line 127A05 and hit At4g14260 are shown.
Line ID 127A05
Locus At4g14260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8216559
Primers used for PCR 10wx - 8474, show primer details
   
Sequence Name 62-K118874-0022-127-A05C-10wx
GenBank Accession LN510864 [GenBank]
Sequencing primer gene specific primer, 10wx: 5'-TCAAGATAAAGATTGGGTAGTGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K118874-0022-127-A05C-10wx
GCCTTTTGGCGCTCTAATGGATCAACATGAAATTCATGTCTCAGTGTATAAACCCTTTAT
CTAGCCTCATGTTCTCCAAGAGAGATCAAAGGTTCTACATTCCAAGTTACGGAGGCAACT
ACTTGTGCTACTTGGATCTCAACTCTAAGGAGGGTGATGATCATTTCAACTCTAGGCAGG
AGAATGGGCAACCCAGCTTCATCGACTTGGATTATGAAAATCTTCCAGAGTCGGTGTTTA
AGCAGTTGGCGGGAGTGAGTTCATGTTCCAAGACAGATCACCTTGTGGAGTCACCCACCG
GCCAACTTTTCCTCGTCAAGTGGTATAGTATCTAATTAACTAGACATCTCTCATAACATA
TTTACAATATATCTTCATTTCGTTGGTGGTTTTTTGGCTTGAGAATCGTATTGGTTCTAT
CATGTCTTTGCAGGTTCGTGGAGGACGTGGAGGACCTTTGCGACAATACACCCATGCACG
TAACAAAAAAGTTCATGGTGTTTAGAGCAAATGAGGCTTCTGACGAANAGAAGAAAAAGA
AAAAGAAAGAGTATGATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCC
   
Sequence Name 22-K118873-0022-127-A05C-8409
GenBank Accession LN496330 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K118873-0022-127-A05C-8409
CTCTTTCTTTTCTTTTTCTTCTTTTCGTCGAAGCCTCATTTGCTCTAAACACCATGAACT
TTTTTGTTACGTGCATGGGTGTATTGTCGCAAAGGTCCTCCACGTCCTCCACGAACCTGC
AAAGACATGATAGAACCAATACGATTCTCAAGCCAAAAAACCACCAACGAAATGAAGATA
TATTGTAAATATGTTATGAGAGATGTCTAGTTAATTAGATACTATACCACTTGACGAGGA
AAAGTTGGCCGGTGGGTGACTCCACAAGGTGATCTGTCTTGGAACATGAACTCACTCCCG
CCAACTGCTTAAACACCGACTCTGGAAGATTTTCATAATCCAAGTCGATGAAGCTGGGTT
GCCCATTCTCCTGCCTAGAGTTGAAATGATCATCACCCTCCTTAGAGTTGAGATCCAAGT
AGCACAAGTAGTTGCCTCCGTAACTTGGAATGTAGAACCTTTGATCTCTCTTGGAGAACA
TGAGGCTAGATAAAGGGTTTATACACTGAGACATGAATTTCATGTTGATCCATTTAGAGC
TGCCAAAAGGCCTGCAAAGACTAACCCGAGATCCCGATAATTTGACACCCACTACCC

Last Updated on 06.12.2018 16:11