Sequences from confirmation amplicon


Confirmation sequences for line 127A06 and hit At4g10270 are shown.
Line ID 127A06
Locus At4g10270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6374996
Primers used for PCR AC57 - 8474, show primer details
   
Sequence Name 50-K104214-0022-127-A06C-AC57
GenBank Accession LN507156 [GenBank]
Sequencing primer gene specific primer, AC57: 5'-CGTTCTTAAAAGGCTAACAACGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K104214-0022-127-A06C-AC57
ACACCAAACCACGGCCACAGCTTCTAGTTTAACTCCAACTACACGGCAATATTGTTGGCT
TCTTCTCTCTTACATAAACACACCAATTCACTTCACTTTCTAAACATCATCAACAAAAAC
AATTCTCAATACACAAAACACAAAACACAAAGAAGTTTAATTCTCTGAAGAAAGATGAGT
TCTACAAGCAAAGCATGGACAGTGGCAGTGAGCATCGGAGCCGTAGAGGCATTAAAAGAC
CAACTAGGTCTTTGTCGGTGGAACTACATACTCCGGTCGGTTAATCAACATCTCCGGAAC
AACGTTAGATCTGTTTCTCAAGGGAAAAGGTTCTCTTCGTCTTCTGTCTCCGCAGCCGTT
ACCTCCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCC
   
Sequence Name 10-K104215-0022-127-A06C-8409
GenBank Accession LN490632 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K104215-0022-127-A06C-8409
TTGGAGGTAACGGCTGCGGAGACAGAAGACGAAGAGAACCTTTTCCCTTGAGAAACAGAT
CTAACGTTGTTCCGGAGATGTTGATTAACCGACCGGAGTATGTAGTTCCACCGACAAAGA
CCTAGTTGGTCTTTTAATGCCTCTACGGCTCCGATGCTCACTGCCACTGTCCATGCTTTG
CTTGTAGAACTCATCTTTCTTCAGAGAATTAAACTTCTTTGTGTTTTGTGTTTTGTGTAT
TGAGAATTGTTTTTGTTGATGATGTTTAGAAAGTGAAGTGAATTGGTGTGTTTATGTAAG
AGAGAAGAAGCCAACAATATTGCCGTGTAGTTGGAGTTAAAACTAGAAGCTGTGGCCGTT
GGTTTTGGTTGTTTACTACTGTCTGGATAGTAAAATGACGTTGTTAGCC

Last Updated on 06.12.2018 16:11