Sequences from confirmation amplicon


Confirmation sequences for line 127F04 and hit At3g45220 are shown.
Line ID 127F04
Locus At3g45220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16566103
Primers used for PCR CD60 - 8474, show primer details
   
Sequence Name 30-K104189-0022-127-F04C-CD60
GenBank Accession LN499698 [GenBank]
Sequencing primer gene specific primer, CD60: 5'-GGCTTCAACATAAGTGAACAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K104189-0022-127-F04C-CD60
ATTGGACATTACTCTTAGGTATCTCACATCTAAATTAAGATGGAACCTAAAAAAAAAATA
TATGTTATAGACCTAATTCGATCATGATTTTTTTTTTTTTTTTGACAGGGTTAGCCATTG
GCAAATAAATTTAAGGTGGGAACCCCAGGAACCCTTGCCCCCAAAAATTTGTTTGGGGGC
AAAAAAAATTTCAAAAAAATTTGCAAAAAACAAAGGGGATTTCCCTGGGTGGCAAATCCA
CTTGGATAACAAGTAGCGTTAAGGGATTTCCCCAAAAAATCTTTAAGGGAGGGTTTGAGG
GAAAAAAATTTGTCATCCCAACCCCCAAAACCCGAAAAAAAGGGCAAGTCCTTTCCCCCC
TTTCCACCTTTTAGGGCAAAAAAAACAACGGCAACCCACAGGAAGGAAATTTCCATTTGA
AAAGGGCTCCTGGCCGGGGAAATCCCCGGGGACCCCCAAGGAGTAGGAGGGCCAATTGGG
AAAAAAAAAAAAAGTATTTCCCATTTTTTTTCCATTTCAAAAAGGAAAATTCCCCCCCCG
TTTTAAAAAAA
   
Sequence Name 46-K104190-0022-127-F04C-8409
GenBank Accession LN505855 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K104190-0022-127-F04C-8409
CCATTTACATTGAATATATCATTCATGTAGATTGCAGTCGTATCTGTTGCCTTAAATGAT
GGAATGGAGAGAAGTGACTTGCACTTATCTACGGCTTATGGTGTATGGATTGACAAATCT
TTATCCTTCAAACCTTCCTTTAAAGATCTTTTGGAGAACTCCTATAACGCTACTTGTAAT
CAAGTTGACTTCGCAACCAAGGTAACTCCTCTATGTTCTTCGCAATTCTTATTGAACTTT
TCTTTGCCTCAAATCAATTATTAGGTGACAAGGTTTCATGAGTTTCCAACTTTAATTTAA
TTAGCAATTGCATAACCTTGTCAAAAAAAAAAAAAAAATCAGGACCAAATTGGGTCTAAA
AAAAATTTTTTTTTTTTGGGTCCCCCCTAATTTAAAAGGGGAAAACCCAAAAAAAAGGGT
CCCATTTCCCCCTTGGGGATGGGAAATTTGTTGTCCCTTT

Last Updated on 06.12.2018 16:11