Sequences from confirmation amplicon


Confirmation sequences for line 127H03 and hit At2g30750 are shown.
Line ID 127H03
Locus At2g30750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13100710
Primers used for PCR MS43 - 8474, show primer details
   
Sequence Name 33-K017401-022-127-H03C-MS43
GenBank Accession LN500792 [GenBank]
Sequencing primer gene specific primer, MS43: 5'-TGAATTTGATGTCGTCTCTTTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K017401-022-127-H03C-ms43
CAATACTCCTCTCGTCTACGATTGATACACAGTATATCGACAAAGTCCTCTTTATGATTA
CCTGCCTCTAAATGTTCTTGCACCACTTTGTCCATAAGATCGCTAAACCCTTGACTTACT
TCCTTTATTCTAGCATTGAAACCGTTGATCCTGTCTATCCATGCCAAAGCCGGGACATAG
TCCCCGATTGGGAACTCGCCTAAAAGCTCCATGATCTGCCTCACTCGCTTCTTGAGATCC
CTTGCGGTTTCGTCCTCACTATGTTTTCTTCCCAAGGCAATTCTACTCGTAACATCGCTT
GGGAGAGTAACAAAGAGTTCGCTCAGATTTTCTGACGAAGAAGAAGAACTATTTTTCTCC
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
TTCGACGTGTCTACATTCA
   
Sequence Name 34-K017401-022-127-H03C-8409
GenBank Accession LN501216 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K017401-022-127-H03C-8409
GGACATGAAGCCATTTACAATTGAATATATCCTGGAGAAAAATAGTTCTTCTTCTTCGTC
AGAAAATCTGAGCGAACTCTTTGTTACTCTCCCAAGCGATGTTACGAGTAGAATTGCCTT
GGGAAGAAAACATAGTGAGGACGAAACCGCAAGGGATCTCAAGAAGCGAGTGAGGCAGAT
CATGGAGCTTTTAGGCGAGTTCCCAATCGGGGACTATGTCCCGGCTTTGGCATGGATAGA
CAGGATCAACGGTTTCAATGCTAGAATAAAGGAAGTAAGTCAAGGGTTTAGCGATCTTAT
GGACAAAGTGGTGCAAGAACATTTAGAGGCAGGTAATCATAAAGAGGACTTTGTCGATAT
ACTGTTATCAATCGAAAGCGAGAAGAGTATTGGATTCCAAGCTCAAAGAGACGA

Last Updated on 06.12.2018 16:11