Sequences from confirmation amplicon


Confirmation sequences for line 128C11 and hit At4g34510 are shown.
Line ID 128C11
Locus At4g34510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16492476
Primers used for PCR AY32 - 8474, show primer details
   
Sequence Name 49-K021645-022-128-C11C-8409
GenBank Accession LN506692 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K021645-022-128-C11C-8409
GCCATTTACAGCTAGTGATATGTTACAAATCCATAGGAACACTTTTGCTCTTGTGGTTAG
TACTGAGAACATCACTCAGAATTGGTATTTTGGTAACAAGAAAGCAATGTTGATCCCTAA
TTGCTTGTTTAGAGTTGGTGGTTCCGCGGTTCTGCTTTCGAACAAGCCTTTGGATCGAAA
ACGATCCAAGTATAAGCTTGTTCATACGGTCAGGACTCATAAAGGATCTGATGAGAACGC
ATTCAATTGTGTGTATCAAGAACAAGATGAGTGTTTGAAAACCGGAGTTTCTTTGTCTAA
AGATCTTATGGCTATAGCTGGAGAAGCTTTAAAGACGAATATCACTTCTTTGGGTCCTCT
GGTTCTTCCTATAAGCGAGCAGATTCTGTTCTTTGCGACTTTTGTTGCTAAGAGATTGTT
CAATGACAAGAAGAAGAAGCCTTACATACCGGATTTCAAGCTTGCTTTAGATCATTT
   
Sequence Name 50-K021645-022-128-C11C-AY32
GenBank Accession LN507037 [GenBank]
Sequencing primer gene specific primer, AY32: 5'-CGCGTGAATACAGAAATGATCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K021645-022-128-C11C-ay32
GTAGGCTTCTTCTTCTTGTCATTGAACAATCTCTTAGCAACAAAAGTCGCAAACAACAGA
ATCTGCTCGCTTATAGGAAGAACCAGAGGACCCAAAGAAGTGATATTCGTCTTTAAAGCT
TCTCCAGCTATAGCCATAAGATCTTTAGACAAAGAAACTCCGGGTTTTCAAACACTCATC
TTGTTCTTGATACACACAATTGAATGCGTTCTCATCAGATCCTTTATGAGTCCTGACCGT
ATGAACAAGCTTATACTTGGATCGTTTTCGATCCAAAGGCTTGTTCGAAAGCAGAACCGC
GGAACCACCAACTCTAAACAAGCAATTAGGGATCAACATTGCTTTCTTGTTACCAAAATA
CCAATTCTGAGTGATGTTCTCAGTACTAACCACAAGAGCAAAAGTGTTCCTATGGATTTG
TAACATATCACTAGCTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA
CGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTA
TTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11