Sequences from confirmation amplicon


Confirmation sequences for line 128D08 and hit At2g34160 are shown.
Line ID 128D08
Locus At2g34160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14426816
Primers used for PCR KH26 - 8474, show primer details
   
Sequence Name 42-K103233-0022-128-D08C-8409
GenBank Accession LN504427 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K103233-0022-128-D08C-8409
GTGCAGCCATTGCAACTGTTGTTACTGTTACTGAAATACTGAACAACAATGGTTTTGCTG
TTGAGAAGAGTAAGTATCTTTATAGTTTCACTATTGGAGATTTCACGACTCTAAGCTTTG
CTTTTTTTTTGACAAATATGGTTTCTTCTTCTATATAGAGATCATGACTTCAACTGTTGA
TATTAAGGATGATGCAAGAGGACGTCCTGTGCAGAAAGCTAAGGTAGTTCTTCATGCCTA
CTACTTGTGAAACTATAATTTTCATCGACCTCTTATCTTGATTTAACAGTTTGGTTTTTG
GTCTTGCAGATTGAGATTACGCTTGTGAAGTCAGAGAAGTTTGATGAACTAATGGCTGCA
GCCAATGAAGAAAAAGAAGATGCAGAAGCTCAGGTGCAGAACTGAAAATAGCTTTTAACT
ATTTTGGGTTTCTTGATGCTTATTCTATAAAGGGCTTTATTTGTTTAATTTCATTAATTT
TTGGCTTGTTATCACAATGGATGGTTTGTTGTATCTGTTAACTTTTGATAGGAAGAATGA
TAGCTAAACAATAAAACATCTCGGCCATAA
   
Sequence Name 90-K103234-0022-128-D08C-KH26
GenBank Accession LN515036 [GenBank]
Sequencing primer gene specific primer, KH26: 5'-ATTTATGGCCGAGATGTTTTATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K103234-0022-128-D08C-KH26
AGTTACAGATACACAAACCATCCATTGTGATAACAAGCCAAAAATTAATGAAATTAAACA
AATAAAGCCCTTTATAGAATAAGCATCAAGAAACCCAAAATAGTTAAAAGCTATTTTCAG
TTCTGCACCTGAGCTTCTGCATCTTCTTTTTCTTCATTGGCTGCAGCCATTAGTTCATCA
AACTTCTCTGACTTCACAAGCGTAATCTCAATCTGCAAGACCAAAAACCAAACTGTTAAA
TCAAGATAAGAGGTCGATGAAAATTATAGTTTCACAAGTAGTAGGCATGAAGAACTACCT
TAGCTTTCTGCACAGGACGTCCTCTTGCATCATCCTTAATATCAACAGTTGAAGTCATGA
TCTCTATATCGAAGAAGAAACCATATTTGTCAAAAAAAAAGCAAAGCTTAGAGTCGTGAA
ATCTCCAATAGTGAAACTATAAAGATACTTACTCTTCTCAACAGCAAAACCATTGTTCTT
CAGTATTTCAGTAACAGTAACAACAGTGGCAATGGCGGCACATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGATAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATT
ATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACTT

Last Updated on 06.12.2018 16:11