Sequences from confirmation amplicon


Confirmation sequences for line 130H01 and hit At1g21610 are shown.
Line ID 130H01
Locus At1g21610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7577476
Primers used for PCR 08dl - 8474, show primer details
   
Sequence Name 20-K109936-0022-130-H01C-08dl
GenBank Accession LN495253 [GenBank]
Sequencing primer gene specific primer, 08dl: 5'-TAAAGTTGCTAGGATTGCGGTACT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K109936-0022-130-H01C-08dl
TTTTTGAACTTGAACGGTATGAGAAAAACTAATATGCCTTTTGTGGCAGCAGGCAAGCCA
GGGAAAACATTCAACAGAGTTAATCAACCGTCTTATGAGCATTGTTGGTCACCTAATCCA
GCTTAGGTCTCTTAAGGTAAAATTTTCTGTCTCCTGTATCCTAGTCTTTCTGTTACTAAA
GCAGACTTGTTAGGACTTTCGTCAAGGTGTTTAAATAGTTAGTCTTCTTCAGGACATTCA
TGTTCCAGTGAAAAACTCTTCTGTTTCAGGTACAAGCAAACAAAATATGGTTTCTTACTC
TTTGTAATGCTTATTTTGCAGAGGAACTTGAAAATCATGATTGATATGGGAGATTCTGCA
ACCCGAGAAAAGGATACTAGGTTCAAGCAAATCAACAATGAAGTTCTTGATATGATAAAG
GCCAAAGTTTCTTTGATGGAATCACAGGTTCTCTCTCTCTCTCTCTGTTCTAATCTTAAG
TTGCTTGATAGGCATTTCTTTCATCCCTGGCTTTTCTATCTTAATACATTCTTGGAAGTG
TCATTAACTTTTATGAAATCTGGAGTTCTCTCTGAAATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACCTGGATCAGCAATGAATATGATGGTCAATATGGAAAAAAAGAAAG
AATAATTACCAATTTTTTTTCAAT
   
Sequence Name 04-K109935-0022-130-H01C-8409
GenBank Accession LN487963 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K109935-0022-130-H01C-8409
GCCATTTACAATTGAATATATCAGAGAGAACTCCAGATTTCATAAAAGTTAATGACACTT
CCAAGAATGTAATAAGATAGAAAAGCCATGGATGAAAGAAATGCCTATCAAGCAACTTAA
GATTAGAACAGAGAGAGAGAGAGAGAACCTGTGATTCCATCAAAGAAACTTTGGCCTTTA
TCATATCAAGAACTTCATTGTTGATTTGCTTGAACCTAGTATCCTTTTCTCGGGTTGCAG
AATCTCCCATATCAATCATGATTTTCAAGTTCCTCTGCAAAATAAGCATTACAAAGAGTA
AGAAACCATATTTTGTTTGCTTGTACCTGAAACAGAAGAGTTTTTCACTGGAACATGAAT
GTCCTGAAGAAGACTAACTATTTAAACACCTTGACGAAAGTCCTAACAAGTCTGCTTTAG
TAACAGAAAGACTAGGATACAGGAGACAGAAAATTTTACCTTAAGAGACCTAAGCT

Last Updated on 06.12.2018 16:11