Sequences from confirmation amplicon


Confirmation sequences for line 131C12 and hit At1g60590 are shown.
Line ID 131C12
Locus At1g60590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22316132
Primers used for PCR 05ve - 8474, show primer details
   
Sequence Name 68-K118051-0022-131-C12C-05ve
GenBank Accession LN512093 [GenBank]
Sequencing primer gene specific primer, 05ve: 5'-TTGAGGACTCTCAACAATTCAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K118051-0022-131-C12C-05ve
GGATGAGTGATGACACTCAGGTACAGACTCAAAAGATTCATAATCCGTTACAAAATTCAA
TCTAGTTGTTCTTGACTAATCAAGAATTTTAATCTCGCTTTCATGAGAATTGTTTCTAAA
AAATAACCATAATGTGAATTACAGTTGAGAAGGTAACACGAGACAGAACAAGAAACAGAG
CATAAAAAAAGTTTTCTCTCTGTTCTGTAAAGTCATAACTTTTTTCTTTTTTCCTCTCAA
TGTTTTAATTAAATTCCCCAATAAACTAACAAACGAGCATAGAGAGAAATAACAACAAAA
ATACCCGATTTAAGAGTGGACAATATTCTCTGTTTTGATTTAAGGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGT
TATTAT
   
Sequence Name 32-K118050-0022-131-C12C-8409
GenBank Accession LN500752 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K118050-0022-131-C12C-8409
TTGAAATATCCTTAAATCAAAACAGAGAATATTGTCCACTCTTAAATCGGGTATTTTTGT
TGTTATTTCTCTCTATGCTCGTTTGTTAGTTTATTGGGGAATTTAATTAAAACATTGAGA
GGAAAAAAGAAAAAAGTTATGACTTTACAGAACAGAGAGAAAACTTTTTTTATGCTCTGT
TTCTTGTTCTGTCTCGTGTTACCTTCTCAACTGTAATTCACATTATGGTTATTTTTTAGA
AACAATTCTCATGAAAGCGAGATTAAAATTCTTGATTAGTCAAGAACAACTAGATTGAAT
TTTGTAACGGATTATGAATCTTTTGAGTCTGTACCTGAGTGTCATCACTCATTCCATCAC
CCTTAGCTCCAAAATCAAGCACATTGAATTGTTGAGGGTTCCTCA

Last Updated on 06.12.2018 16:11