Sequences from confirmation amplicon


Confirmation sequences for line 131D04 and hit At3g18040 are shown.
Line ID 131D04
Locus At3g18040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6175205
Primers used for PCR DE10 - 3144, show primer details
   
Sequence Name 29-K019339-022-131-D04C-3144
GenBank Accession LN499093 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K019339-022-131-D04C-3144
TCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGG
ACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGCATT
TTTCATTTACACCGCAGTGGAATTATCTTACTCTGTGGCAACTTACCTTATATACGTGAT
CTGAATTTTTTTTTAGAAGTAGTAGAAATGCTACATTGTTCATTTGGTTTCTTCTTGATT
ATGTTTCCTTTGCATCGGCTCTTAACTGGTTTATGATTCCGTATAGTCTCACCCTGAATC
TATGGTACCTTATAAAGTCTAGAAGATACATATGATCTGTAGAGTTTGGAGAACTAGGCA
AAATTCTTCTCAGTTTTCA
   
Sequence Name 30-K019339-022-131-D04C-DE10
GenBank Accession LN499529 [GenBank]
Sequencing primer gene specific primer, DE10: 5'-ATGAAAACTGAGAAGAATTTTGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K019339-022-131-D04C-DE10
CAGATCATATGTATCTTCTAGACTTTATAAGGTACCATAGATTCAGGGTGAGACTATACG
GAATCATAAACCAGTTAAGAGCCGATGCAAAGGAAACATAATCAAGAAGAAACCAAATGA
ACAATGTAGCATTTCTACTACTTCTAAAAAAAAATTCAGATCACGTATATAAGGTAAGTT
GCCACAGAGTAAGATAATTCCACTGCGGTGTAAATGAAAAATGCACTGATAGTTTAAACC
GAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACT
TCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTC
AGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTC
CACGATGCTCCTCGTGGGTGGGGGTCCATCTTTG

Last Updated on 06.12.2018 16:11