Sequences from confirmation amplicon


Confirmation sequences for line 131G01 and hit At3g05280 are shown.
Line ID 131G01
Locus At3g05280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1505138
Primers used for PCR BW24 - 8474, show primer details
   
Sequence Name 03-K104268-0022-131-G01C-BW24
GenBank Accession LN487470 [GenBank]
Sequencing primer gene specific primer, BW24: 5'-AAATCTCATTGGACCGGAGTAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K104268-0022-131-G01C-BW24
AGAGATCGAACGGAAGATCGGAACAGATGATGTCCGGCGGGAACTACACGACTATAGACA
GCCAGAAAGTCTCTGGATCTGTACCTGTAAGTTTTTTATGTCTCGATCTCGAATGTTTAC
ATGAGTGCTCGTCCCTACACTGATTCTTCCGATCTGATCTAGATTTGTTTGTGTCAATCG
TAGTCTGTTCCAGATCCAGGCCATACCACCGTCAAATTCGCAGGTAACATTCTCGTTTTT
ATGATTTTTGGTTGCTGAGGTGAAATTGATATTAGAGCGTTTAGGGAAAGAACGATTTTA
TTTGGAATTATAGGCCTTTTTTTTTCTCTGGGACGTAAGACTATGTTGAATCTTAGCTAA
AATTACTGCAATTTGTTAGGTAATGGCTATGGATTTTGAATTAAACTGTAGTTTGAACAG
ATGAGGGTTTAGCCAATAGTAGATAAATATTTTATGCTTTGATAATTTTGATGAGGGAAA
TATTTGATCCATAATTTACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCA
   
Sequence Name 19-K104269-0022-131-G01C-8409
GenBank Accession LN494742 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K104269-0022-131-G01C-8409
AGCCTTTACATTGTAATTATGGATCAATATTTCCCTCATCAAAATTATCAAAGCATAAAA
TATTTATCTACTATTGGCTAAACCCTCATCTGTTCAAACTACAGTTTAATTCAAAATCCA
TAGCCATTACCTAACAAATTGCAGTAATCTTAGCTAAGATTCAACATAGTCTTACGTCCC
AGAGAAAAAAAAAGGCCTATAATTCCAAATAAAATCGTTCTTTCCCTAAACGCTCTAATA
TCAATTTCACCTCAGCAACCAAAAATCATAAAAACGAGAATGTTACCTGCGAATTTGACG
GTGGTATGGCCTGGATCTGGAACAGACTACGATTGACACAAACAAATCTAGATCAGATCG
GAAGAATCAGTGTAGGGACGAGCACTCAGGTAAACATTCGAGATCGAGACATAAAAAACT
TACAGGTACAGATCCAGAGACTTTCTGGCTGTCTATAGTCGTGTAGTTCCCGCCGGACAT
CATCTTGTTCCGATCTTCCGTTCGATTCTCTCTGAAGAAACGATATTGGCCTTGAAGAAA
TCTATACNACTCCGGTCCAATGAGAT

Last Updated on 06.12.2018 16:11