Sequences from confirmation amplicon


Confirmation sequences for line 131G02 and hit At3g62430 are shown.
Line ID 131G02
Locus At3g62430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23094208
Primers used for PCR 14yk - 8474, show primer details
   
Sequence Name 87-K120200-0022-131-G02C-14yk
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 14yk: 5'-TTTTTAAAACGCAACTGGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K120200-0022-131-G02C-14yk
ATTTTATAAGGGAGCACGTTTTGTTGAGGGAAGTTTCTCCGTCTGTTCACGGATGGAATA
TTCCACCAAGATGCCTCCTCAGAAAATATCAAATATTATTGCCAAGTGTTAATTAACTGA
ATATTATATATTTCTGAATTTGAAAATTTCCTCACGAAAGCAACGTCTAACACACAATTC
ATAAGCGTAATTATAAAACGACCATTAACTATAACGAAAGTCTACCAGATTGTTGTTTAT
ATGCGGCTCTCTTTGGTTAAAACTTAAATGATGACGTGACGATTACATCATTTTATTGAC
ACGATTTTAAATTACAAAACACAAGACTGAGTTGGGAAGTTGGCTCGTGGGCTTTATAAT
AGCCTTAATTCAGCTGATGGGCTTAGCTCTCCCTTACACACTCGAAAAATCCAGTTAGAG
ATAAGAAAAACGCCAAGCTTATGTCAACAAAAGTTTGATCTTTTTAAGATTACTTCTCTC
ACAATCTTCACAAACTAGATTCTGAGCTTATGCAAAGATTTAAGAGTGTGATACAAAAAC
AAACTCAGTTCTCTCATGTCAATAAATGGGATATGTTAAGATTTTGTTCAAGAGGGCTTG
ATACGAAGTTTGCATTTAGATGAATTTCTCGGTAGCATTAGGAGATCCAACATGATCTGA
ATCTTTGTGTCAGCTCTTATTATTGGCTTAAGCTGAACTTCCAAAAGCTCAAGACTTGGC
AATTTCTCTAACATGTGCTTTATTTGCACCATTTCTCCAACAGTTCTCATGTTGAGGCAA
CAAACACTTGTTTGTTCAAACAAACTTGACCTGGACTTTGCATTTGGATGAAGCTCTCGG
AAGCATTAGGAGATCCGCCAAGATCTGCAGCTTTCTGTCAATCTTCTTTGTCTTAACATG
   
Sequence Name 69-K120199-0022-131-G02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K120199-0022-131-G02C-8409
CATTTACAATTGAAAAATCTACAAATCTAAAGACTCTCACCATTGAGGTGACCAAAAACC
CCAAAAGTTCATTGGACAAACAAAAGAATTGAAACTCACAAAATCTTCAATCTCAAAATT
CCGGTCTTTTTGCAGGCTTTGCATTATCATGGCTATGGGGATGAAAACTCTGTTTGCGAA
TGCTTGGATGGGTATTCTTTCCTTTTGTCCTGCCCTATTGAGATCCTAAAGATAACTGAG
TTCGGTGGAGAGATTGAAGAAATGGAGCAAGTAGAGTATGTCTTGGAGAATTTGCTGTGT
CTGGTGCTGTTGGAAATTCATGTTAAGACAAAGAAGATTGACAGAAAGCTGCAGATCTTG
GCGGATCTCCTAATGCTTCCGAGAGCTTCATCCAAATGCAAAGTCCAGGTCAAGTTTGTT
TGAACAAACAAGTGTTTGTTGCCTCAACATGAGAACTGTTGGAGAAATGGTGCAAATAAA
GCACATGTTAGAGAAATTGCCAAGTCTTGAGCTTTTGGAAGTTCAGCTTAAGCCAATAAT
AAGAGCTGACACAAAGATTCAGATCATGTTGGATCTCCTAATGCTACCGAGAAATTCATC
TAAATGCAAACTTCGTATCAAGCCCTCTTGAACAAAATCTTAACATATCCCATTTATTGA
CATGAGAGAACTGAGTTTGTTTTTGTATCACACTCTTAAATCTTTGCATAAGCTCAGAAT
CTAGTTTGTGAAGATTGTGAGAGAAGTAATCTTAAAAAGATCAAACTTTTGTTGACATAA
GCTTGGCGTTTTTCTTATCTCNTACTGGATTTTTCGAGTGTGTAAGGGAGAGCTAAGCCC
ATCAGCTGAATTAAGGCTATTATAAAGCCCACGAGCCAACTTCCCAACTCAGTCTTGTGT
TTTGTAATTAAAATCGTGTCAATAAAATGATGTAATCGTCACGTCATCATTTA

Last Updated on 06.12.2018 16:11