Sequences from confirmation amplicon


Confirmation sequences for line 131G09 and hit At4g33400 are shown.
Line ID 131G09
Locus At4g33400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16079511
Primers used for PCR 01gj - 3144, show primer details
   
Sequence Name 66-K112062-0022-131-G09C-01gj
GenBank Accession LN511642 [GenBank]
Sequencing primer gene specific primer, 01gj: 5'-GTCTAATTTTGCCATCAAGAGAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K112062-0022-131-G09C-01gj
CAAGCACTGAAAGTTAGTCCCTCTAGAAAACTGATGCCCCTGTGCCCAATTCAAGACTGG
AGTATTCGCAGTAGCAAGATCCTGAACCATCCCATATCGGTCACGCATATCCCACCTGCA
AAGCCTATTGTTATCCAGACCAAGAAACGTTGACGCAGATGGATCCAATTGAGCACCTTT
ACCATCATTAGTAATGTCACTCATAGAGATATCCACTCCATCTTTCTCAAACTTCCACTC
TGAAATAATTTTCCCGGTTTCAATGTCAAGCTGATGGATACCTCGTGAATGAGGCGTTTG
GCTCATAGGACTCATGAGCAGCATATTAGTCTCAGCTCTCATGAGAAGAGCCTTCTTCGG
TGCTGAAGAATGAGTTCGACCATAACCAGGCTCAAAGTTAACACAAACACCTTTCAATTG
TAAATGTAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTA
CCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATA
GTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATC
   
Sequence Name 87-K112061-0022-131-G09C-3144
GenBank Accession LN514781 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K112061-0022-131-G09C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTACAT
TTACAATTGAAAGGTGTTTGTGTTAACTTTGAGCCTGGTTATGGTCGAACTCATTCTTCA
GCACCGAAGAAGGCTCTTCTCATGAGAGCTGAGACTAATATGCTGCTCATGAGTCCTATG
AGCCAAACGCCTCATTCACGAGGTATCCATCAGCTTGACATTGAAACCGGGAAAATTATT
TCAGAGTGGAAGTTTGAGAAAGATGGAGTGGATATCTCTATGAGTGACATTACTAATGAT
GGTAAAGGTGCTCAATTGGATCCATCTGCGTCAACGTTTCTTGGTCTGGATAACAATAGG
CTTTGCAGGTGGGATATGCGTGACCGATATGGGATGGTTCAGGATCTTGCTACTGCGAAT
ACTCCAGTCTTGAATTGGGCACAGGGGCATCAGTTTTCTAGAGGGACTAACTTTCAGTGC
TTTGCAACTACTGGTGATGGCTCANTTGTTGTTGGCTCTCTNGATGGCAAAATTAGA

Last Updated on 06.12.2018 16:11