Sequences from confirmation amplicon


Confirmation sequences for line 132C04 and hit At1g54730 are shown.
Line ID 132C04
Locus At1g54730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20424771
Primers used for PCR 20pm - 8474, show primer details
   
Sequence Name 36-K115064-0022-132-C04C-20pm
GenBank Accession LN502338 [GenBank]
Sequencing primer gene specific primer, 20pm: 5'-AAAATGAGAGGAGAAATCGATGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K115064-0022-132-C04C-20pm
CAAGAGATCAAGACTCATCGGCGACATAACAACTACTCTCTTGCTCACCACTTTTGTAGC
CGTGTCTGGTTCCTTTGTCTTTGGCTCAGCTGTGAGTTTACAATTACTAATTTTTGCTTT
TGTTCAGTAATTGTGTGTTGTGTTTTGTGTTTGAATCTTAATTTCTAATTTCGTTGAAAA
TTTTGGCAGATAGGATATTCATCACCTGTTCAGTCGGATTTAACCAAGGAGTTGAATCTC
TCTGTATGATTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCACGATAATT
   
Sequence Name 04-K115063-0022-132-C04C-8409
GenBank Accession LN488032 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K115063-0022-132-C04C-8409
TTGAAAATCATACAGAGAGATTCAACTCCTTGGTTAAATCCGACTGAACAGGTGATGAAT
ATCCTATCTGCCAAAATTTTCAACGAAATTAGAAATTAAGATTCAAACACAAAACACAAC
ACACAATTACTGAACAAAAGCAAAAATTAGTAATTGTAAACTCACAGCTGAGCCAAAGAC
AAAGGAACCAGACACGGCTACAAAAGTGGTGAGCAAGAGAGTAGTTGTTATTGTCGCCGA
TGAGTCTTGATTCTCTTTGTTAATAAGTGAAGTTTCAGGAGCAAGATTAGCTTCATCGAT
TTCTCTTCTCATTT

Last Updated on 06.12.2018 16:11