Sequences from confirmation amplicon


Confirmation sequences for line 132C11 and hit At1g24490 are shown.
Line ID 132C11
Locus At1g24490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8684959
Primers used for PCR RK30 - 8474, show primer details
   
Sequence Name 35-K015898-022-132-C11C-8409
GenBank Accession LN501618 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K015898-022-132-C11C-8409
ATAGATTTCTCCGACATGAAGCCATTTACAATTGAATATATCCTGTTCTCTGTTTCATGA
GAATGCTGTTGTTCTGTATCATGGCCAATGCCGAATGTCCCATTCGTAGTCTCGTCCTTT
TGAATGGATGGCTTCCCATTCACAGCTACTGTGCCATCGGATGTTTCATCCACAGCAGAA
AGTTCTGTTTTTTTATCATCTTCAGCGACAATGGCTGTATCAGATTTTTCATCTTGTTCA
TGTGCTTTGTCTGTATTTTGATTAGATAGAGCTGCTTCAGCTTTCTGCCTCTCTTCTTTT
TCTCGACGTCTCTTTGCTTCTTGCTCTTTCAGCAGCCTAAACCTGGGATCACATGGACGT
GTTAGTACAAAAAAACAGCTTCTAGGAACGAATGATGACACATTTTAGAAGATTTGGTTA
CCTTTCACCAGGTTTGGGGCATTCTGGGGTCACCTTTTCACCACCCTTCTCTCTTGGTAT
TTTCAGTTCTGAAACAGACTTCTGAACTAGTGGTTCTGAAAAAGACTTCTCAATTTGTTG
TGTTTTATCTTCCTTAGTGACCAAATTAGTGAATTTCTCCACTGGATTCTTAGCACCACC
ATATTTTTGAAGCCATACTTGCTGTGCCGTGCTCAAAATGTTGTTGGTCAGCCTGCAAAA
ACACTTTAAA
   
Sequence Name 36-K015898-022-132-C11C-RK30
GenBank Accession LN502025 [GenBank]
Sequencing primer gene specific primer, RK30: 5'-CTTCCACTCGCTATTCTTCTCAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K015898-022-132-C11C-rk30
GTTTTTGCAGGCTGACCAACAACATTTTGAGCACGGCACAGCAAGTATGGCTTCAAAAAT
ATGGTGGTGCTAAGAATCCAGTGGAGAAATTCACTAATTTGGTCACTAAGGAAGATAAAA
CACAACAAATTGAGAAGTCTTTTTCAGAACCACTAGTTCAGAAGTCTGTTTCAGAACTGA
AAATACCAAGAGAGAAGGGTGGTGAAAAGGTGACCCCAGAATGCCCCAAACCTGGTGAAA
GGGTAACCAAATCTTCTAAAAGGGGTCATCTTTCGTTCCTAAAAGTTGTTTTTTTGTACT
ACCCCGTCCTGGGGATCCCAGGTTAAGGTGGTGAAAGAGCAAGAAGCAAAGAGACGTCGA
GAAAAAGAAGAGAGGCAGAAAGCTGAAGCAGCTCTATCTAATCAAAATACAGACAAAGCA
CCTGGACCAGATGGAAAA
Primers used for PCR HV19 - 8474, show primer details
   
Sequence Name 07-K031753-022-132-C11C-8409
GenBank Accession LN489152 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K031753-022-132-C11C-8409
CATTTACAATTGAATATATCCTGTTCTCTGTTTCATGAGAATGCTGTTGTTCTGTATCAT
GGCCAATGCCGAATGTCCCATTCGTAGTCTCGTCCTTTTGAATGGATGGCTTCCCATTCA
CAGCTACTGTGCCATCGGATGTTTCATCCACAGCAGAAAGTTCTGTTTTTTTATCATCTT
CAGCGACAATGGCTGTATCAGATTTTTCATCTTGTTCATGTGCTTTGTCTGTATTTTGAT
TAGATAGAGCTGCTTCAGCTTTCTGCCTCTCTTCTTTTTCTCGACGTCTCTTTGCTTCTT
GCTCTTTCAGCAGCCTAAACCTGGGATCACATGGACGTGTTAGTACAAAAAAACAGCTTC
TAGGAACGAATGATGACACATTTTAGAAGATTTGGTTACCTTTCACCAGGTTTGGGGCAT
TCTGGGGTCACCTTTTCACCACCCTTCTCTCTTGGTATTTTCAGTTCTGAAACAGACTTC
TGAACTAGTGGTTCTGAAAAAGACTTCTCAATTTGTTGTGTTTTATCTTCCTTAGTGACC
AAATTAGTGAATTTCTCCACTGGATTCTTAGCACCACCATATTTTTGAAGCCATA
   
Sequence Name 08-K031753-022-132-C11C-HV19
GenBank Accession LN489618 [GenBank]
Sequencing primer gene specific primer, HV19: 5'-AGTATGGCTTCAAAAATATGGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K031753-022-132-C11C-HV19
ATTTGGTCACTAAGGAAGATAAAACACAACAAATTGAGAAGTCTTTTTCAGAACCACTAG
TTCAGAAGTCTGTTTCAGAACTGAAAATACCAAGAGAGAAGGGTGGTGAAAAGGTGACCC
CAGAATGCCCCAAACCTGGTGAAAGGTAACCAAATCTTCTAAAATGTGTCATCATTCGTT
CCTAGAAGCTGTTTTTTTGTACTAACACGTCCATGTGATCCCAGGTTTAGGCTGCTGAAA
GAGCAAGAAGCAAAGAGACGTCGAGAAAAAGAAGAGAGGCAGAAAGCTGAAGCAGCTCTA
TCTAATCAAAATACAGACAAAGCACATGAACAAGATGAAAAATCTGATACAGCCATTGTC
GCTGAAGATGATAAAAAAACAGAACTTTCTGCTGTGGATGAAACATCCGATGGCACAGTA
GCTGTGAATGGGAAGCCATCCATTCAAAAGGACGAGACTACGAATGGGACATTCGGCATT
GGCCATGATACAGAACAACAGCATTCTCATGAAACAGAGAACAGGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAG
GCGAAAGCAATAAACAAATTATTCTAATTCGGAGATCTTTATTTCGACGTGTCTACATTC
ACT

Last Updated on 06.12.2018 16:11