Sequences from confirmation amplicon


Confirmation sequences for line 132F04 and hit At3g47090 are shown.
Line ID 132F04
Locus At3g47090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17343380
Primers used for PCR NR74 - 3144, show primer details
   
Sequence Name 72-K102507-0022-132-F04C-NR74
GenBank Accession LN512704 [GenBank]
Sequencing primer gene specific primer, NR74: 5'-GGCTTTAGTTTCAGTTCCTTGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K102507-0022-132-F04C-NR74
ACTAAGTAGCATTTTGAATATTCCTTCTGTTGGGACTCTTCCCTCGAAATTGTTATCGGA
TAGATTGAGATATTCCAACTTGGAAAAGTTTTCAAAATATTCAGATATACTTCCAGAGAG
ATTATTGTTTGACAAATCAACGTTTTTAACACCCATCAACCCTTTTATATCTGGAATGGT
TCCATCAAAATGGTTTTCTTGTAGATAAATAACTTCCATCGAAAGACATTTTCCCAAAGT
TTGTGGGAGATGTCCTGATAAGTTATTATTCCCAAGTAATAGCTCAACAAGATTTTGTAA
TCTTCCAATATCGTTTGGTAGAGAGCCACTCAAAGAATTAGATTCCATGTTGAGGTGAAC
AAGGGTTGGGATTTGCATGATCTCCTTAGGTATAGTCCCATTTAACTTATTATACCCAAT
CTGTAAGTCGAGCATGTGACTACAATCACCAAGACTCGGAGGAACGATTCCTTCAAAACT
ATTGTTGGATAAATATAGTTTTACTAACTGAGTAAGGTTACACTGATAGTTTAAACCGAA
GGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCC
TTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGT
GGAGATATCACATCAATCCACTTGCTTTGAAGACATGGTTGGAACGTCTTCTTTTTC
Primers used for PCR 62wr - 8474, show primer details
   
Sequence Name 69-K118656-0022-132-F04C-62wr
GenBank Accession LN512288 [GenBank]
Sequencing primer gene specific primer, 62wr: 5'-TGGGATATAATCATCTGGAAGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K118656-0022-132-F04C-62wr
TGGTGTCTCTTACATTAACAATGAAAACTTCTCTGGCGTTTTTCCTCCTGCTTTCTACAA
CTTGTCCTCACTTGAGAATTTATACCTCCTTGGTAATGGTTTCTCGGGTAACTTGAAGCC
TGATTTTGGTAATCTGCTACCAAACATTCATGAGTTATCTTTGCATGGAAATTTTCTAAC
AGGAGCCATCCCAACAACACTTGCCAATATTTCAACTCTGGAAATGTTTGGAATTGGTAA
AAACAGGATGACAGGAAGTATCTCTCCCAACTTTGGAAAACTAGAGAATTTGCATTACCT
AGAACTTGCTAACAATTCTTTGGGAAGTTACTCTTTTGGAGATCTTGCATTTCTTGATGC
TTTGACTAATTGCTCCCATTTGCATGGGTTATCTGTGAGTTACAATAGGCTTGGGGGTGC
TTTGCCTACATCCATTGTCAATATGTCCACGGAACTCACCGTTTTGAACCTTAAAGGAAA
CCTCATTTATGGAAGCATTCCTCATGATATTGGAAATCTCATAGGCCTACAATCACTTTT
GTTGGCAGATAATCTATTAACAGGACCACTCCCAACCTCTCTTGGAAATCTTGTAGGGCT
CGGGGAATTAATTTTGTTTTCAAATAGGTTTTCTGGAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 43-K118655-0022-132-F04C-8409
GenBank Accession LN505008 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K118655-0022-132-F04C-8409
GCCATTTCCGAAACCTATTTGAAAACAAAATTAATTCCCCGAGCCCTACAAGATTTCCAA
GAGAGGTTGGGAGTGGTCCTGTTAATAGATTATCTGCCAACAAAAGTGATTGTAGGCCTA
TGAGATTTCCAATATCATGAGGAATGCTTCCATAAATGAGGTTTCCTTTAAGGTTCAAAA
CGGTGAGTTCCGTGGACATATTGACAATGGATGTAGGCAAAGCACCCCCAAGCCTATTGT
AACTCACAGATAACCCATGCAAATGGGAGCAATTAGTCAAAGCATCAAGAAATGCAAGAT
CTCCAAAAGAGTAACTTCCCAAAGAATTGTTAGCAAGTTCTAGGTAATGCAAATTCTCTA
GTTTTCCAAAGTTGGGAGAGATACTTCCTGTCATCCTGTTTTTACCAATTCCAAACATTT
CCAGAGTTGAAATATTGGCAAGTGTTGTTGGGATGGCTCCTGTTAGAAAATTTCCATGCA
AAGATAACTCATGAATGTTTGGTAGCAGATTACCAAAATCAGGCTTCAAGTTACCCGAGA
AACCATTACCAAGGAGGTATAAATTCTCAAGTGAGGACAAGTTGTAGAAAGCAGGAGGAA
AAACGCCAGAGAAGTTGTTCATTGTTAATGTAAGAGACACCATTTGACTCAGCATAGCTA
TATCATCTGGAATTTCTCCTTCCAGATATAAATATCCCAAGTTGGAGCACTATTAGAGA

Last Updated on 06.12.2018 16:11