Sequences from confirmation amplicon


Confirmation sequences for line 133E09 and hit At3g61910 are shown.
Line ID 133E09
Locus At3g61910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22929847
Primers used for PCR MZ17 - 8474, show primer details
   
Sequence Name 03-K017253-022-133-E09C-MZ17
GenBank Accession LN487270 [GenBank]
Sequencing primer gene specific primer, MZ17: 5'-AGTCACAAGTACCTCCTGGCTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K017253-022-133-E09C-MZ17
CTCTTGAAGTATTACCTCCGCAAGAAAATCTCTAACATCAAGATCGATCTCGATGTTATT
CCTGACATTGATCTCAACAAGCTCGAGCCTTGGGATATTCAAGGTTCATCATTTATCTTT
TATTCTTGATGAAACGTATTAATAGGGCTTGTATATATTAATTAAATTAGTTTTTTAATG
CATGTGTTGAGTCAAGAACTAAAGCTCTGAATTCAGGAAATTTTGCAGAGTAATATATAC
TTTTTGTGATAATAAAATTTGCAGTAACAAAATTCAGTCAAAACTTAACACAACTCTTGT
TTTTTCTATGTAGAGATGTGTAAGATTGGAACGACGCCGCAAAACGATTGGTACTTTTAT
AGCCATAAGGACAAGAAGTATCCCACCGGGACTAGAACCAACAGAGCCACCACGGTCGGA
TTTTGGAAAGCGACGGGACGTGACAAGACCATATATACCAATGGTGATAGAATCGGGATG
CGAAAGACGCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
AT
   
Sequence Name 04-K017253-022-133-E09C-8409
GenBank Accession LN487713 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K017253-022-133-E09C-8409
GACATGAAGCCATTTACAATTGAAGCGTCTTTCGCATCCCGATTCTATCACCATTGGTAT
ATATGGTCTTGTCACGTCCCGTCGCTTTCCAAAATCCGACCGTGGTGGCTCTGTTGGTTC
TAGTCCCGGTGGGATACTTCTTGTCCTTATGGCTATAAAAGTACCAATCGTTTTGCGGCG
TCGTTCCAATCTTACACATCTCTACATAGAAAAAACAAGAGTTGTGTTAAGTTTTGACTG
AATTTTGTTACTGCAAATTTTATTATCACAAAAAGTATATATTACTCTGCAAAATTTCCT
GAATTCAGAGCTTTAGTTCTTGACTCAACACATGCATTAAAAAACTAATTTAATTAATAT
ATACAAGCCCTATTAATACGTTTCATCAAGAATAAAAGATAAATGATGAACCTTGAATAT
CCCAAGGCTCGAGCTTGTTGAGATCAATGTCAGGAATAACATCGAGATCGATCTTGATGT
TAGAGATTTTCTTGCGGAGGTAATACTTCAAGAGCTCTTCCTCGGTTGGGTGAAACCTAA
AGCCAGGAGG

Last Updated on 06.12.2018 16:11