Sequences from confirmation amplicon


Confirmation sequences for line 133H11 and hit At1g50780 are shown.
Line ID 133H11
Locus At1g50780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18821286
Primers used for PCR AC70 - 8474, show primer details
   
Sequence Name 06-K104226-0022-133-H11C-AC70
GenBank Accession LN488791 [GenBank]
Sequencing primer gene specific primer, AC70: 5'-CTCCATGAAGATGGTTCCATAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K104226-0022-133-H11C-AC70
TAGAGTGTTGCAGTGAAGCTGAGAAGGAAGATGCTGATGAAAGGATCAATGAGAAGGTGC
TAGCATTAAAGGCAGACAGAAAAATACCAAGCCCTCATCAAAAGTTTGCTTCTGGAATCT
GGATGCACTAATGGAGATGAAACAAGAAAATCATATAAGCATAAGGTTTTGAAGCCATCT
GATGAGTCAAACATTCACATCGCTGTAGGGGATCGAAATGAGGTACAGGATTGTTTGCTT
CATGATGAACAAATCTACATGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 22-K104227-0022-133-H11C-8409
GenBank Accession LN496092 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K104227-0022-133-H11C-8409
GCCATTTACAATTGAATATATCCTGACATGTAGATTTGTTCATCATGAAGCAAACAATCC
TGTACCTCATTTCGATCCCCTACAGCGATGTGAATGTTTGACTCATCAGATGGCTTCAAA
ACCTTATGCTTATATGATTTTCTTGTTTCATCTCCATTAGTGCATCCAGATTCCAGAAGC
AAACTTTTGATGAGGGCTTGGTATTTTTCTGTCTGCCTTTAATGCTAGCACCTTCTCATT
GATCCTTTCATCAGCATCTTCCTTCTCAGCTTCACTGCAACACTTCTTATCTGATCCCAC
TATTTCTCTAGTTATGGAACCATCTNTCATGGA

Last Updated on 06.12.2018 16:11