Sequences from confirmation amplicon


Confirmation sequences for line 134D03 and hit At1g76590 are shown.
Line ID 134D03
Locus At1g76590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28741504
Primers used for PCR AC61 - 8474, show primer details
   
Sequence Name 45-K104214-0022-134-D03C-AC61
GenBank Accession LN505519 [GenBank]
Sequencing primer gene specific primer, AC61: 5'-GAAGAAACATACCTCCTTCAAACG-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K104214-0022-134-D03C-AC61
GAAACTATTTCAATCCCTTTGAGTACAGTATATACCTAATTTCTATATGTACTATAAAAA
GAAAAACCAAACAATACCTGTAAGTAATATGTCTTACAATATGTATATTCCTAGCTCAAA
AGGTTAAAAACTACTAACCATTTAGTTACAACATAACAACCACATTTTCTATAATCTACA
CTATATAATTAGCTTCCGTTAGTATTAATTTTAGAAAGGAGCACGGTGTGGGACGCCCTT
CCGTCTACTTGAATTCCGGTGATTATATATCGGCGGTGTCCCCGGAGAAAGTTTAAAACC
GCTCTCATCACCGGAGCTCGAAGACGGTTGATCTCCTGACAAGTAATCACATTTAACGGT
GGAGATGGAAAGACCACTCATCAGACGGTTGAAAGCACATGTCTTGCGTATCTTAGTTGG
TGTAGTCGCTTCATCAGATTCCCACTCACCTTCGTACTCTCTTCCATGCTTCCCTCTCAA
AGAGAAGGTTAGACTTGGATCACGCTTCATTCCTCCAAGCTGAAATAAACCAAACAAAAG
GGCAATATGGTCATTTCAACTATACAGAGCAAATTCAGATATTCATTCAAAACAGAGGAA
AACAGAGGAAAGAAGAGTTACTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 05-K104215-0022-134-D03C-8409
GenBank Accession LN488333 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K104215-0022-134-D03C-8409
TTTCCTCTGTTTTGAATGAATATCTGAATTTGCTCTGTATAGTTGAAATGACCATATTGC
CCTTTTGTTTGGTTTATTTCAGCTTGGAGGAATGAAGCGTGATCCAAGTCTAACCTTCTC
TTTGAGAGGGAAGCATGGAAGAGAGTACGAAGGTGAGTGGGAATCTGATGAAGCGACTAC
ACCAACTAAGATACGCAAGACATGTGCTTTCAACCGTCTGATGAGTGGTCTTTCCATCTC
CACCGTTAAATGTGATTACTTGTCAGGAGATCAACCGTCTTCGAGCTCCGGTGATGAGAG
CGGTTTTAAACTTTCTCCGGGGACACCGCCGATATATAATCACCGGAATTCAAGTAGACG
GAAGGGCGTCCCACACCGTGCTCCTTTCTAAAATTAATACTAACGGAAGCTAATTATATA
GTGTAGATTATAGAAAATGTGGTTGTTATGTTGTAACTAAATGGTTAGTAGTTTTTAACC
TTTTGAGCTAGGAATATACATATNGTAAGACATATTACTTACAGGTATTGTTTGGTTTTT
CTTTTTATAGTACATATAGAAATTAGGTATATACTGTACTCAAAGGGATTGAAATATGTT
TTCATATAGTTTTTTTGCTGNTAATATACGTTTGAAGG

Last Updated on 06.12.2018 16:11