Sequences from confirmation amplicon


Confirmation sequences for line 134E10 and hit At4g39400 are shown.
Line ID 134E10
Locus At4g39400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18326435
Primers used for PCR MR26 - 8474, show primer details
   
Sequence Name 31-K015315-022-134-E10C-8409
GenBank Accession LN499937 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K015315-022-134-E10C-8409
ATCTCGCTATCCTCAAGCTAAGCAACAATTCATTCTCCGGGAACATTCCGGCTGAGCTCG
GCGACTGCAGAAGCTTAATCTGGCTTGATCTCAACACCAATCTCTTCAATGGAACGATTC
CGGCGGCGATGTTTAAACAATCCGGGAAAATCGCTGCCAATTTCATCGCCGGTAAGAGGT
ACGTTTATATCAAAAACGATGGGATGAAGAAAGAGTGTCATGGAGCTGGTAATTTACTTG
AGTTTCAAGGAATCAGATCCGAACAATTAAACCGGCTTTCAACGAGGAACCCTTGTAATA
TCACTAGCAGAGTCTATGGAGGTCACACTTCGCCGACGTTTGATAACAATGGTTCGATGA
TGTTTCTGGACATGTCTTACAACATGTTGTCTGGATACATACCGAAGGAGATTGGTTCGA
TGCCTTATCTGTTTATTCTCAATTTGGGTCATAACGATATCTCTGGTTCGATTCCTGATG
AGGTAGGTGATCTAAGAGGTTTAAACATTCTTGATCTTTCAAGCAATAAGCTCGATGGGA
GGATTCCTCAGGCTATGTCAGCTCTTAC
   
Sequence Name 32-K015315-022-134-E10C-MR26
GenBank Accession LN500361 [GenBank]
Sequencing primer gene specific primer, MR26: 5'-CCGTAAGCATAGTAAGAGCTGACA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K015315-022-134-E10C-MR26
GAATCCTCCCATCGAGCTTATTGCTTGAAAGATCAAGAATGTTTAAACCTCTTAGATCAC
CTACCTCATCAGGAATCGAACCAGAGATATCGTTATGACCCAAATTGAGAATAAACAGAT
AAGGCATCGAACCAATCTCCTTCGGTATGTATCCAGACAACATGTTGTAAGACATGTCCA
GAAACATCATCGAACCATTGTTATCAAACGTCGGCGAAGTGTGACCTCCATAGACTCTGC
TAGTGATATTACAAGGGTTCCTCGTTGAAAGCCGGTTTAATTGTTCGGATCTGATTCCTT
GAAACTCAAGTAAATTACCAGCTCCATGACACTCTTTCTTCATCCCATCGTTTTTGATAT
AAACGTACCTCTTACCGGCGATGAAATTGGCAGCGATTTTCCCGGATTGTTTAAACATCG
CCGCCGGAATCGTTCCATTGAAGAGATTGGTGTTGAGATCAAGCCAGATTAAGCTTCTGC
AGTCGCCGAGCTCAGCCGGAATGTTCCCGGAGAATGAATTGTTGCTTAGCTTGAGGATAG
CGAGATTCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTTATTTC

Last Updated on 06.12.2018 16:11