Sequences from confirmation amplicon


Confirmation sequences for line 134G02 and hit At3g01150 are shown.
Line ID 134G02
Locus At3g01150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:52699
Primers used for PCR 09vf - 8474, show primer details
   
Sequence Name 59-K117914-0022-134-G02C-09vf
GenBank Accession LN510098 [GenBank]
Sequencing primer gene specific primer, 09vf: 5'-TGATTTTACGATTTCGAGTGTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K117914-0022-134-G02C-09vf
GGATTGGTTGTTTTTGATGAAAATTGTTGAGATTGATGGTTTTAGATGGGTGTGGCAATA
CATGCGGTAGTGTGTTGTGCTTCGAGCAGATGAATATATAAAAATTTCTAATCAAGCAAA
CTTCTACGAGTAATTTTTACTTAGATATACTATTCCAATTACAGTATATCAACATTAATG
AAATGCTCGAATCACAGATGTTGATGCCTCTTTATTAAAATCTTTCTGGAGAGAGTTTGG
AAATTGTATCGTCATAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAAAATTATA
   
Sequence Name 19-K117913-0022-134-G02C-8409
GenBank Accession LN494947 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K117913-0022-134-G02C-8409
TCATTATGACGATACAATTTCCAAACTCTCTCCAGAAAGATTTTAATAAAGAGGCATCAA
CATCTGTGATTCGAGCATTTCATTAATGTTGATATACTGTAATTGGAATAGTATATCTAA
GTAAAAATTACTCGTAGAAGTTTGCTTGATTAGAAATTTTTATATATTCATCTGCTCGAA
GCACAACACACTACCGCATGTATTGCCACACCCATCTAAAACCATCAATCTCAACAATTT
TCATCAAAAACAACCAATTCCCACAGAGAAATACCCATTGATTAAAAAAAATCAGTGAAA
GAAGCTCACACTCGAAATGGTAAAATCAAGGGCAAAGAAANACCCCTTGCTCCTTTTTTT
TCCTTGAAAAAAGCTCACACTCGCCATCAAAAAAAAAAAA

Last Updated on 06.12.2018 16:11