Sequences from confirmation amplicon


Confirmation sequences for line 135A10 and hit At3g03640 are shown.
Line ID 135A10
Locus At3g03640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:883446
Primers used for PCR VL11 - 8474, show primer details
   
Sequence Name 27-K017368-022-135-A10C-VL11
GenBank Accession LN498164 [GenBank]
Sequencing primer gene specific primer, VL11: 5'-TATGATCGATTAAACAGAGTTGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K017368-022-135-A10C-vl11
TCTGATCTTCTATTCCTCTTTCCTTGAATCCGAATCTACCTTCGACTGAATCGTGTCCGG
GACTTTCTCAAGGAGCTTGGAGAGCCACTTGGCAGATTGTTTCTCATGCCGTCCCATATT
GTGTCCATAGTCTACATAGTACAGTCCAGACCTCGTCTTGTATCCATCTTCCCACTCTAG
TCCATCCATAAGCGACGACACAAAGTAACCTTTAACATTAACCTTGTCCTCGCTGCAAAA
ACCAAAAAAGTTTTAGATATGAAGCAGAAACAGTTTTTATTTTTATTTTTTATCACTAAT
TCCAGCAACTAGAGAAGAAGAAACAGAGTTTTTTGGAACCGTGAAAAGTAACTTACCAGA
CTGCTCCATGTAATGCCATGAGATGTCTCATATGATAATACTTCCTGTTACTATCGCTGA
GGGCATCTGGAAGTACATCTTTCTCTTCAAGTGTCTCCTTGTACCCTGCAAATGTAATGA
ATTTAGTTTCTTGCAAAGGAAATCTGAGTGAATGAATCTTTAAGCAGAGAGCTACAAAGC
CAATGGCTTACCGTTTCCAGTGACTATGATTTCTGGGTCATTGTAGTTCTCTACATGGAA
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAA
   
Sequence Name 31-K017368-022-135-A10C-8409
GenBank Accession LN499945 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K017368-022-135-A10C-8409
GCCATTTACAATTGAATATTTCCATGTAGAGAACTACAATGACCCAGAAATCATAGTCAC
TGGAAACGGTAAGCCATTGGCTTTGTAGCTCTCTGCTTAAAGATTCATTCACTCAGATTT
CCTTTGCAAGAAACTAAATTCATTACATTTGCAGGGTACAAGGAGACACTTGAAGAGAAA
GATGTACTTCCAGATGCCCTCAGCGATAGTAACAGGAAGTATTATCATATGAGACATCTC
ATGGCATTACATGGAGCAGTCTGGTAAGTTACTTTTCACGGTTCCAAAAAACTCTGTTTC
TTCTTCTCTAGTTGCTGGAATTAGTGATAAAAAATAAAAATAAAAACTGTTTCTGCTTCA
TATCTAAAACTTTTTTGGTTTTTGCAGCGAGGACAAGGTTAATGTTAAAGGTTACTTTGT
GTCGTCGCTTATGGATGGACTAGAGTGGGAAGATGGATACAAGACGAGGTCTGGACTGTA
CTATGTAGACTATGGACACAATATGGGACGGCATGAGAAACAATCTGCCAAGTGGCTCTC
CAAGCTCCTTGAGAAAGTCCCGGACACGATTCAGTCGAAGGTAGATTCGGATTCAAGGAA
AGAGTTATAAATGATCAGAGATCCTTTTCGCAACTCTG

Last Updated on 06.12.2018 16:11