Sequences from confirmation amplicon


Confirmation sequences for line 135C12 and hit At2g22360 are shown.
Line ID 135C12
Locus At2g22360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9500062
Primers used for PCR 11kh - 8474, show primer details
   
Sequence Name 70-K113549-0022-135-C12C-11kh
GenBank Accession LN512424 [GenBank]
Sequencing primer gene specific primer, 11kh: 5'-TTTTTCTCCTCTTTGCTCAATCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K113549-0022-135-C12C-11kh
ATCTCCTCTCAATACTCTTGTTCATACCGGAACTCCTTTTTTCGCCATCACAAGCGTCGT
GCTAGGTTGTGTCCCAGCTGGAACTTTCAAATCTACTGTCCCATCCACTGTTGGTACTTT
CAGTGTCGTCCCTAAAATCGCATCGATATACGATATCTTGCAAGTGTAGAGAATATTTGT
ATCGTCTCGTTTCAAAATCGGGTCTGGGATAACTTCTATAACAACAAACAGATCGCCTGG
TGATCCGCCTCTCTTCCCGGCATTGCCTTCTCCTCTCATGATATACAACAAACGGCTTCA
TGACCATCATATTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTATA
   
Sequence Name 05-K113548w-0022-135-C12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K113548w-0022-135-C12C-8409
GAATATATCCTGAATATGATGGTCATGAAGCCGTTTGTTGTATATCATGAGAGGAGAAGG
CAATGCCGGGAAGAGAGGCGGATCACCAGGCGATCTGTTTGTTGTTATAGAAGTTATCCC
AGACCCGATTTTGAAACGAGACGATACAAATATTCTCTACACTTGCAAGATATCGTATAT
CGATGCGATTTTAGGGACGACACTGAAAGTACCAACAGTGGATGGGACAGTAGATTTGAA
AGTTCCAGCTGGGACACAACCTAGCACGACGCTGGTGATGGCGAAAAAAGGAGTTCCGGT
ATTGAACAAGAGTAATATGAGAGGAGATCAGTTGGTGAGAGTACAAGTGGAGATACCTAA
GAGATTGAGCAAAGAGGAGAAAA

Last Updated on 06.12.2018 16:11