Sequences from confirmation amplicon


Confirmation sequences for line 135E10 and hit At1g04160 are shown.
Line ID 135E10
Locus At1g04160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1096137
Primers used for PCR AS15 - 8474, show primer details
   
Sequence Name 15-K023370w-022-135-E10C-AS15
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, AS15: 5'-CGTTTTTGCCTAGACTCCATACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K023370w-022-135-E10C-AS15
GGCTTCTTGTTTCACTTTATTACAGATCCTTAGTGTCCAACAACTTTACCGGATATGCAC
ATTATACTGGGACGACTGCTATAACACCCGAAGTGTGTCTCAAGAGGTAATATCGAGCAT
GCGTGCGCTCATGACAGAAGAATCCAACGATGCCGACAGCAATTCCTTCTTGTTGGATGA
TAATTCCAGGTAACTCGAATCATTTCTTAAAGATTAATATCAGTAGTAGAAACTAAAACC
GGTTTATTGTTCGCAGCATTCCCTTCTCAATAGACGAGATTTCGAATTCGATGCACGAAA
AAGACTTTGCGAGTGTCAAACCGGCCAAAGAGCTACTGGAAAACCCAGAATTCGTATTCC
CAGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
TTCGACGTGTCTACATT
   
Sequence Name 11-K023370w-022-135-E10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K023370w-022-135-E10C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCTGGGAATACGAATT
CTGGGTTTTCCAGTAGCTCTTTGGCCGGTTTGACACTCGCAAAGTCTTTTTCGTGCATCG
AATTCGAAATCTCGTCTATTGAGAAGGGAATGCTGCGAACAATAAACCGGTTTTAGTTTC
TACTACTGATATTAATCTTTAAGAAATGATTCGAGTTACCTGGAATTATCATCCAACAAG
AAGGAATTGCTGTCGGCATCGTTGGATTCTTCTGTCATGAGCGCACGCATGCTCGATATT
ACCTCTTGAGACACACTTCGGGTGTTATAGCAGTCGTCCCAGTATAATGTGCATATCCGG
TAAAGTTGTTGGACACTAAGGATCTGTAATAAAGTGAAACAAGAAAGCCATTAAGTATGG
AGTCT

Last Updated on 06.12.2018 16:11