Sequences from confirmation amplicon


Confirmation sequences for line 135G01 and hit At3g53020 are shown.
Line ID 135G01
Locus At3g53020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19660902
Primers used for PCR 03gg - 8474, show primer details
   
Sequence Name 31-K111953-0022-135-G01C-03gg
GenBank Accession LN500222 [GenBank]
Sequencing primer gene specific primer, 03gg: 5'-ATAGGAGGACAAGAGAGAGCAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K111953-0022-135-G01C-03gg
GCAGCAATTCTCAGTCTGATATTATAGAGTAGCAAAAGTGAGAGACAAAAACGAAATAAC
AAGGTTTATGTTTTGCAAATATTCAGAAATGAACATCAACTACTAAAAGTAGCTACTAGC
AGAAAAGACGCAGAGTGAGAAAAGATGGCTTTAAGCTCTTCAGCGTTTGCCACCACCACC
TCCCACCTTAGGACCCTTGGATGCAGCAGCAGCTTTGGGGAAATTAGCCTTGACCTTCTG
TTGCTTAGAAGCAAATTCCACCTTCTTAGCCTTCTTTTCATCTTTGGTCTTTTTGATTCT
TTCCTTGATCTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCA
   
Sequence Name 15-K111952-0022-135-G01C-8409
GenBank Accession LN493033 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K111952-0022-135-G01C-8409
ATTGAGATCAGGAAAGAATCAAAAAGACCAAAGATGAAAAGAAGGCTAAGAAGGTGGAAT
TTGCTTCTAAGCAACAGAAGGTCAAGGCTAATTTCCCCAAAGCTGCTGCTGCATCCAAGG
GTCCTAAGGTGGGAGGTGGTGGTGGCAAACGCTGAAGAGCTTAAAGCCATCTTTTCTCAC
TCTGCGTCTTTTCTGCTAGTAGCTACTTTTAGTAGTTGATGTTCATTTCTGAATATTTGC
AAAACATAAACCTTGTTATTTCGTTTTTGTCTCTCACTTTTGCTACTCTTATAATATCAG
ACTTGAGAATTTTGCTGCTTATGAACTTTATTTCACTGCTCTCTCTTGTCCTCCTA

Last Updated on 06.12.2018 16:11