Sequences from confirmation amplicon


Confirmation sequences for line 135G05 and hit At1g58360 are shown.
Line ID 135G05
Locus At1g58360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21677605
Primers used for PCR CL58 - 8474, show primer details
   
Sequence Name 76-K033098-022-135-G05C-8409
GenBank Accession LN513329 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K033098-022-135-G05C-8409
CTTTACATTGAATATGGCGGATTGTACTGCTTAAAATAGGCGTATCACGAGGCCCTTTCG
TCTCGCGCGTTTCGGTGATGACAATCTGAATGGCTTCCAACTGCTTCAATTGTTTTTAAT
TGTTTGTGGATAGAATTGAAGATCCAATTGTTTGTGGATAGAATTGCTGATCATTACAAG
TATGATATCCACAAACATCATACTCATTGCTGATCCAATTGTTTGTGGATAGAATTGAAG
ATGTAATAAATGGAAATTTCTTATTTTCTGTGTTTCCTTTAAATCCCTGCAATTTCAGAG
GAAAAAACTGTGGATTGTAATTGCCTCAAATTGCTCTATTTCTATTTTAAACATAAATAT
TTTGGCTAGATATAAAACTTTGGTAACCGTCCATTTAATTCCCAATTATACACTATGGTC
TATGTTTGTTGAAATAATTAATTAGGCTGAAACTTTATCTTAGTCAATCAATAATATCTT
TAGCTGGTTGTCAAACTAAATTTACTAATTAAATTTTGGACTGTTTAT
   
Sequence Name 28-K033098-022-135-G05C-CL58
GenBank Accession LN498738 [GenBank]
Sequencing primer gene specific primer, CL58: 5'-ACAATTTGGCTCAATAAACAGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K033098-022-135-G05C-CL58
GATATTATTGATTGACTAAGATAAAGTTTCAGCCTAATTAATTATTTCAACAAACATAGA
CCATAGTGTATAATTGGGAATTAAATGGACGGTTACCAAAGTTTTATATCTAGCCAAAAT
ATTTATGTTTAAAATAGAAATAGAGCAATTTGAGGCAATTACAATCCACAGTTTTTTCCT
CTGAAATTGCAGGGATTTAAAGGAAACACAGAAAATAAGAAATTTCCATTTATTACATCT
TCAATTCTATCCACAAACAATTGGATCAGCAATGAGTATGATGTTTGTGGATATCATACT
TGTAATGATCAGCAATTCTATCCACAAACAATTGGATCTTCAATTCTATCCACAAACAAT
TAAAAACAATTGAAGCAGTTCGAAGCCATTCAGATTGTCATCACCGAAACGCGCGAGACG
AAAGGGCCTCGTGATACGCCTATTTTTAAGCAGTACAATCCGCCATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACNAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTC

Last Updated on 06.12.2018 16:11