Sequences from confirmation amplicon


Confirmation sequences for line 135H05 and hit At4g02690 are shown.
Line ID 135H05
Locus At4g02690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1185695
Primers used for PCR CD59 - 8474, show primer details
   
Sequence Name 36-K104189-0022-135-H05C-CD59
GenBank Accession LN502208 [GenBank]
Sequencing primer gene specific primer, CD59: 5'-GTGGTTGATCAAGTCATACCATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K104189-0022-135-H05C-CD59
ATCCACACAAAGCCGAGCCAAGTGAAAATCCCCAAGAGCAAGTAATTCACCGGATGTTTC
TGATGGTAATAGTACAACGGACACAACACTATACATCGGAGAGAAACACAAATCCAAGTT
TCAGCTTTTGAGATTATTACCAAACTTCGAAAAAGATTGAAACTTTTGATTTGTAAAACA
AGTGTGCGTGATGATTGTACCTATGAGAGGTGTGATGATGATGACTATGTAAAGAGCTAA
TCCAAGTCCGGTGGTGGCGAAGAACAGAGCGATTGGACGGACGGTGACCACCGTGGCAGC
GACAGCGACGGTGGCTAGAAGCTGAAAGGCGATTATAGAGTAGACCTTGCGTATAAATCC
CCACCGGAGCTCGGGATTTTCATGCATCGCTGGGTATAGTAAAGGCCTTCTTGAGCTAAC
GCCTGTTTCCACGTCATCTTTCCGGTACGGTAAGTTCCACTGATACATTATTTACGATAG
CAGAGTCTTTATAAAGTGAAGGAACGCGCGGGAAAATTCGAATCCTTTCGATTACTACTG
TATCACGATCTACAGAGAGAGAACCACAAGCGAGAGAGAAAAGTTGATGCGGTGCTTTGC
TTGAGCTTCTTCGGATTTTCCTGGGAGAAGCGGTGATGCCAAGAGGAAGAAGAAAGTTCA
TGAAAGCTCTCGGCTAAACCGTAGATGACGCGTGGCAACCGGAGATGTCGCATAAGCGGT
GACAAGGATTTGATGTGTCCAAAGGGAAAGTGTTCTTTTTTTTGGCTCAACACAATACTT
GCTTAACCAAACTTCAATTGTAAATATATATATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGNCATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCATTCAAAATGT
   
Sequence Name 52-K104190-0022-135-H05C-8409
GenBank Accession LN507803 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K104190-0022-135-H05C-8409
TTCGGACTGAGCCTATATATATTTACATTGAAGTTTGGTTAAGCAAGTATTGTGTTGAGC
CAAAAAAAAGAACACTTTCCCTTTGGACACATCAAATCCTTGTCACCGCTTATGCGACAT
CTCCGGTTGCCACGCGTCATCTACGGTTTAGCCGAGAGCTTTCATGAACTTTCTTCTTCC
TCTTGGCATCACCGCTTCTCCCAGGAAAATCCGAAGAAGCTCAAGCAAAGCACCGCATCA
ACTTTTCTCTCTCGCTTGTGGTTCTCTCTCTGTAGATCGTGATACAGTAGTAATCGAAAG
GATTCGAATTTTCCCGCGCGTTCCTTCACTTTATAAAGACTCTGCTATCGTAAATAATGT
ATCAGTGGAACTTACCGTACCGGAAAGATGACGTGGAAACAGGCGTTAGCTCAAGAAGGC
CTTTACTATACCCAGCGATGCATGAAAATCCCGAGCTCCGGTGGGGATTTATACGCAAGG
TCTACTCTATAATCGCCTTTCAGCTTCTAGCCACCGTCGCTGTCGCTGCCACGGTGGTCA
CCGTCCGTCCAATCGCTCTGTTCTTCGCCACCACCGGACTTGGATTAGCTCTTTACATAG
TCATCATCATCACACCTCTCATAGGTACAATCATCACGCACACTTGTTTTACAAATCAAA
AGTTTCAATCTTTTTCGAAGTTTGGTAATAATCTCAAAAGCTGAAACTTGGATTTGTGTT
TCTCTCCGATGTATAGTGTTGTGTCCGTTGTACTATTACCATCAGAAACATCCGGTGAAT
TACTTGCTCTTGGGGATTTTCACTTTGGCTCTGGCTTTTGTTGTTGGATTAACATGTGCC
TTCACCAATGGTATGACT

Last Updated on 06.12.2018 16:11