Sequences from confirmation amplicon


Confirmation sequences for line 135H07 and hit At1g04140 are shown.
Line ID 135H07
Locus At1g04140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1076368
Primers used for PCR 04kn - 8474, show primer details
   
Sequence Name 74-K113669-0022-135-H07C-04kn
GenBank Accession LN513113 [GenBank]
Sequencing primer gene specific primer, 04kn: 5'-CAAATTCTTCCCTGTCTAGCATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K113669-0022-135-H07C-04kn
TAATTTTTCCAGGATGGAAATCGAAACATTCATATGTTAGTTCAAAAGATTCGTTTGCTT
TCATTCATCTTTTATTATTATTTCAAGAAGATCAACCTTGTTAATAATTCTACCTCGGCC
AAGTTCTCATCGGGAGAGCTATTTGGTGCAGTGAGATTGGACGCAGCAGCTGTGTTGTAC
CGGAAGATCCTTAGTTTTCCCTCCTGAGATAAATATTGAGCAAATATCAATACAAGTCAA
CATTTTAGCGCCGAGAAGTTTTTCACATTACGATTTAGGATTAATTACCTTTGTTCCATA
GACAAGACCTCCCCCAGGAGAAGGATGAAAGCAAGCAACATTAACTTCATCCTCTGAGCT
TGGAAGTACTCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 43-K113666-0022-135-H07C-8409
GenBank Accession LN504906 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K113666-0022-135-H07C-8409
ATGAGTTATGTTGCTTGCTTTCATCCTTCTCCTGGGGGAGGTCTTGTCTATGGAACAAAG
GTAATTAATCCTAAATCGTAATGTGAAAAACTTCTCGGCGCTAAAATGTTGACTTGTATT
GATATTTGCTCAATATTTATCTCAGGAGGGAAAACTAAGGATCTTCCGGTACAACACAGC
TGCTGCGTCCAATCTCACTGCACCAAATAGCTCTCCCGATGAGAACTTGGCCGAGGTAGA
ATTATTAACAAGGTTGATCTTCTTGAAATAATAATAAAAGATGAATGAAAGCAAACGAAT
CTTTTTGAACTAACATATGAATGTTTCGATTTCCATCCTTGGAAAAATTTTGAACCACAG
GTGCAGACCTATGCGTTAGAATGCTAGACAGGGAAGAATTT

Last Updated on 06.12.2018 16:11