Sequences from confirmation amplicon


Confirmation sequences for line 137D04 and hit At1g20930 are shown.
Line ID 137D04
Locus At1g20930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7293814
Primers used for PCR 14sm - 3144, show primer details
   
Sequence Name 47-K116549-0022-137-D04C-14sm
GenBank Accession LN506311 [GenBank]
Sequencing primer gene specific primer, 14sm: 5'-ATGACTCTGATTCTTCCTGCAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K116549-0022-137-D04C-14sm
ATCTAGTTTCAGTTTGCTATTTGTTGTGTGGTCCGATCGAGATAAAATGGGTTCCTTATC
TTATCCGAATACTAGAAAATGTCAAAGTAGAAGCTCTGTGTTGTGATCTCAGTTTCAACT
ATTTTTGTGGTGATTGCATTTCTACTGAGAGATTATTGTTATGATGATTATCTTCTTCTA
CAGGTTGATGGATGTTAAGCAAGGAATAAACAAAGAAGGAAAAACTGTACTTTACCTTGT
TTTCGAGTATGTTGATACTGATCTCAAGAAATTCATCAGAAGCTTTCGTCAAGCTGGACA
GAACATTCCACAAAATACTGTCAAGGTTTGGATATTTGGAGATTTTGTTCTGCTTTTTTT
CTCTCATCCGATAAGCCCCTTTAAGCTAAGAGAAACATGTTCTTGAATTGCAGTGCTTGA
CGATTTAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTAC
CCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAG
TGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATAT
   
Sequence Name 07-K116548-0022-137-D04C-3144
GenBank Accession LN489436 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K116548-0022-137-D04C-3144
TTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCG
GGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTAAATCGTCAAGCA
CTGCAATTCAAGAACATGTTTCTCTTAGCTTAAAGGGGCTTATCGGATGAGAGAAAAAAA
GCAGAACAAAATCTCCAAATATCCAAACCTTGACAGTATTTTGTGGAATGTTCTGTCCAG
CTTGACGAAAGCTTCTGATGAATTTCTTGAGATCAGTATCAACATACTCGAAAACAAGGT
AAAGTACAGTTTTTCCTTCTTTGTTTATTCCTTGCTTAACATCCATCAACCTGTAGAAGA
AGATAATCATCATAACAATAATCTCTCAGTAGAAATGCAATCACCACAAAAATAGTTGAA
ACTGAGATCACAACACAGAGCTTCTACTTTGACATTTTCTAGTATTCGGATAAGATAAGG
AACCCATTTTATCTCGATCGGACCACACAACAAATAGCAAACTGAAACTAAGATCTACCA
GTAACACCTAAGAGCGACACTAAGCGCGATTTGCA

Last Updated on 06.12.2018 16:11