Sequences from confirmation amplicon


Confirmation sequences for line 137D11 and hit At5g42770 are shown.
Line ID 137D11
Locus At5g42770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17152424
Primers used for PCR 10vf - 8474, show primer details
   
Sequence Name 60-K117914-0022-137-D11C-10vf
GenBank Accession LN510362 [GenBank]
Sequencing primer gene specific primer, 10vf: 5'-CGATATTCAAGTTCAGCAATCTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K117914-0022-137-D11C-10vf
TTCAGGTCCGCTTCTTTTCATAGGTTTCTAGTTCGATTCATGAACTGTTTCTGCAGTCAA
TTTCGAACTTGTGAAATCTCCATAACTTGTGCTTTGAATGTATTTGCTAAAGCTATAATC
TTGTTGCGTTTCAAAAACCATCCTTTGGAAAAATTGGAGTCTTTTGGTTGCTTATTGAAA
TTTGTTTGCAGCTAATTTTGGGATCTTCTTCGATTGCTCGACGTAAGATACTAACAGATA
TGGGATACCAATTCATATCTGTTAGTATCTTCAATTGAATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 20-K117913-0022-137-D11C-8409
GenBank Accession LN495400 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K117913-0022-137-D11C-8409
GCCATTTCAATTGAATATTCAATTGAAGATACTAACAGATATGAATTGGTATCCCATATC
TGTTAGTATCTTACGTCGAGCAATCGAAGAAGATCCCAAAATTAGCTGCAAACAAATTTC
AATAAGCAACCAAAAGACTCCAATTTTTCCAAAGGATGGTTTTTGAAACGCAACAGGATT
ATAGCTTTAGCAAATACATTCAAAGCACAAGTTATGGAGATTTCGCAAGTTCGAAATTGA
CTGCAGAAACAGTTCATGAATCGAACTAGAAACCTATTGAAAAGAAGCGGACCTTGAAGT
GAGGATGATTCGATTCCATTTTCTACTGCAATGAGATTGCTGAACTTGAATATCGACGAA
ACTAAAGAGGAGTTTCCCTTTTTTTTTTTTTTCACAAAAGTAAATTTC

Last Updated on 06.12.2018 16:11