Sequences from confirmation amplicon


Confirmation sequences for line 137H09 and hit At4g15740 are shown.
Line ID 137H09
Locus At4g15740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8965117
Primers used for PCR ML16 - 8474, show primer details
   
Sequence Name 26-K100819-0022-137-H09C-8409
GenBank Accession LN497836 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K100819-0022-137-H09C-8409
GCCATTTCAATTGATCAGGTGACGCGTATGGCTGAGGCGATACCGGCACATACATAATAT
ATGGCTGGCCTGGTGTAGATGGTGCTGTAACTTGTTCTGCAAGAAACCTATACGTGAAGC
TCACGACACCTTTTTTTCCATAAGGACCCGTCAAAGCATGTGTCTCCAACTTCATACCGT
TAGCATCACCATTGTCGGTCAACGGATAAGGCGGATTTGAGACCAATAACTGTTGCATCG
GAAGTCTAACAAATCCAATCTCCTTATCACCAAGGAAAGGCCGGTGGCTCATCAACTCCA
CAAGGAGCATCAACCGCCCTTCTTGAGCTGACTTCTCATCAATAGAAAATTTCATCATTT
GATTCCACTTTGGATTTGTATAAGCGGCAAAAGCCACCGGTGTGTTGATCCTATTCTTAA
CTTTTCTGTCCTTNAGAATCGC
   
Sequence Name 26-K100831-0022-137-H09C-ML16
GenBank Accession LN497838 [GenBank]
Sequencing primer gene specific primer, ML16: 5'-TGCGATTCTTAAGGACAGAAAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K100831-0022-137-H09C-ML16
CTTTGCCGCTATACAATCCAAGTGGAATCAAATGATGAAATTTTCTATTGATGAGAAGTC
AGCTCAAGAAGGGCGGTTGATGCTCCTTGTGGAGTTGATGAGCCACCGGCCTTTCCTTGG
TGATAAGGAGATTGGATTTGTTAGACTTCCGATGCAACAGTTATTGGTCTCAAATCCGCC
TTATCCGTTGACCGACAATGGTGATGCTAACGGTATGAAGTTGGAGACACATGCTTTGAC
GGGTCCTTATGGAAAAAAAGGTGTCGTGAGCTTCACGTATAGGTTTCTTGCAGAACAAGT
TACAGCACCATCTACACCAGGCCAGCCATATATTATGTATGTACCGGTATCGCCTCAGCC
ATACGCGTCACCTGATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11