Sequences from confirmation amplicon


Confirmation sequences for line 138B05 and hit At4g15360 are shown.
Line ID 138B05
Locus At4g15360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8771774
Primers used for PCR 10qv - 8474, show primer details
   
Sequence Name 64-K115779-0022-138-B05C-10qv
GenBank Accession LN511267 [GenBank]
Sequencing primer gene specific primer, 10qv: 5'-TAAGGATGCTTTCACAAAGGTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K115779-0022-138-B05C-10qv
ATTATCTATTGGTGAGAACAGAGATGAAATGGTGAAATAAGATTTTGTGTCACAAAAAAA
ATCCAAAGAACTTAAATAAGCTAGATTGAAAAATGATGATGTCGTAGATCCCGTGTATAG
GAATGTCAAATTTTCAGATATAACAAACATAAAGAGATCAAATATAATTCAACGTTTCCG
AGCAAACATATTAAACGTCAAATAGCAATGCAAATACAAAGACAACATAGAAGAGAGCAA
ATGCAAACATATAGAACAAAAGAAAGTAGACTTGTTAACAAGCTCCAAAGCTAAGTATCA
GACACGGAAATTTAGAGATTAAAAGCAAAAGATAAAGGTTGGGTTCGAAGAACCGGAGTG
CAAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCA
CACGGTTATT
   
Sequence Name 16-K115778-0022-138-B05C-8409
GenBank Accession LN493568 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K115778-0022-138-B05C-8409
TCCGGTTCTTCGAACCCAACCTTTATCTTTTGCTTTTATCTCTAAATTTCCGTGTCTGAT
ACTTAGCTTTGGAGCTTGTTAACAAGTCTACTTTCTTTTGTTCTATATGTTTGCATTTGC
TCTCTTCTATGTTGTCTTTGTATTTGCATTGCTATTTGACGTTTAATATGTTTGCTCGGA
AACGTTGAATTATATTTGATCTCTTTATGTTTGTTATATCTGAAAATTTGACATTCCTAT
ACACGGGATCTACGACATCATCATTTTTCAATCTAGCTTATTTAAGTTCTTTGGATTTTT
TTTGTGACACAAAATCTTATTTCACCATTTCATCTCTGTTTCTTCACCAATAGATTAATT
CCACAGAGGCATCGTTTTATATTAAAGAACCTTTGTGAAAGCATCCTT

Last Updated on 06.12.2018 16:11