Sequences from confirmation amplicon


Confirmation sequences for line 138C08 and hit At5g10290 are shown.
Line ID 138C08
Locus At5g10290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3235557
Primers used for PCR ZZ04 - 8474, show primer details
   
Sequence Name 08-K020243-022-138-C08C-8409
GenBank Accession LN489528 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K020243-022-138-C08C-8409
TAGATTTCCCGGACATGAAGCCATTTAGTTCATCGTGACTTCCACGTTTTGCCACTCTTC
CCATCTCTCCGCAAGCCCTTCTCCTTCTAACATCCTCACAACTTCAGACATCACTGGTCG
GTCTTCTGGTGAACCTTGTGTACAAAGCAAAGCCACTTGTATCATCATCTCTACTTCTTC
TTTTATATACTCTCCATCCAAATTCTTATCTACGATTGCTCCTAATCTCTTCTCTCTTTC
CAGTTTCTTCACCTGTCCAAAACAAAAAAAAGATCCAAAAGATTACGGTCAATAAAGCTT
GAGAGAAGCTACTTCGAGGTAGGATTATTTGATTATACATACGTGGTCAAGTAACAAGAC
ATCATCTTCTTCCTCCAAACGTGAAAAGTCTATTGCGCGTTGTCCTGTAACAAGCTCAAG
AAGCATAATTCCATACCCGAAAACATCGGTTCTCTCTGATGATTTCCCTGTTGATAAATA
TTCTGGTGCAATGTGACCCATTGTTCCTCGAACTTGAGTAGTCACATTAGTCCTTCTAAC
ATCTACTAGCTTGGCTA
   
Sequence Name 12-K020243-022-138-C08C-ZZ04
GenBank Accession LN491372 [GenBank]
Sequencing primer gene specific primer, ZZ04: 5'-TGATTTTGGTTTAGCCAAGCTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K020243-022-138-C08C-ZZ04
ATGTTAGAAGGACTAATGTGACTACTCAAGTTCGAGGAACAATGGGTCACATTGCACCAG
AATATTTATCAACAGGGAAATCATCAGAGAGAACCGATGTTTTCGGGTATGGAATTATGC
TTCTTGAGCTTGTTACAGGACAACGCGCAATAGACTTTTCACGTTTGGAGGAAGAAGATG
ATGTCTTGTTACTTGACCACGTATGTATAATCAAATAATCCTACCTCGAAGTAGCTTCTC
TCAAGCTTTATTGACCGTAATCTTTTGGATCTTTTTTTTGTTTTGGACAGGTGAAGAAAC
TGGAAAGAGAGAAGAGATTAGGAGCAATCGTAGATAAGAATTTGGATGGAGAGTATATAA
AAGAAGAAGTAGAGATGATGATACAAGTGGCTTTGCTTTGTACACAAGGTTCACCAGAAG
ACCGACCAGTGATGTCTGAAGTTGTGAGGATGTTAGAAGGAGAAGGGCTTGCGGAGAGAT
GGGAAGAGTGGCAAAACGTGGAAGTCACGATGAACTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCT

Last Updated on 06.12.2018 16:11