Sequences from confirmation amplicon


Confirmation sequences for line 138C12 and hit At1g52190 are shown.
Line ID 138C12
Locus At1g52190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19436393
Primers used for PCR 11de - 8474, show primer details
   
Sequence Name 50-K109676-0022-138-C12C-11de
GenBank Accession LN507198 [GenBank]
Sequencing primer gene specific primer, 11de: 5'-GTGGTAACATTGCAGTTAGCCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K109676-0022-138-C12C-11de
ATCACATGATCATTAATTAATTTACTTTGGTCTATAAATCACTATTTAATTTGAGCTGGA
AGCTAAAAAAACATTACATGTCGTTACACTAACAAACATTACATGCTACGTTTCTATATC
CTTAATTACAGAAAAACTTCCAAGAAGAAAAATCCATTATTCATATCGGCAACAAACTCG
TGAACATGAGCACACGTTGGTAGGTCGAATGTAAAGTCATATGTCACTAACCCTACCAAA
ATTATACTAAATTTTGTTTGATTGAGATGAATAATTGTACCAACATGTCAAATCAATCCA
TGTAACGAAATTGAAACATTTGATTTGCATTATCGACTTCGCCCAAGAATCGGACAAGTT
GTAAAAAGTATGGACACGGTTCAAAAATAATTGCTTAAGCCATAAAAGTTGTAATAAGTA
CGGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGC
   
Sequence Name 34-K109675-0022-138-C12C-8409
GenBank Accession LN501451 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K109675-0022-138-C12C-8409
CATTTACAATTGAATATATCCTGTCCGTACTTATTACAACTTTTATGGCTTAAGCAATTA
TTTTTGAACCGTGTCCATACTTTTTACAACTTGTCCGATTCTTGGGCGAAGTCGATAATG
CAAATCAAATGTTTCAATTTCGTTACATGGATTGATTTGACATGTTGGTACAATTATTCA
TCTCAATCAAACAAAATTTAGTATAATTTTGGTAGGGTTAGTGACATATGACTTTACATT
CGACCTACCAACGTGTGCTCATGTTCACGAGTTTGTTGCCGATATGAATAATGGATTTTT
CTTCTTGGAAGTTTTTCTGTAATTAAGGATATAGAAACGTAGCATGTAATGTTTGTTAGT
GTAACGACATGTAATGTTTTTTTAGCTTCCAGCTCAAATTAAATAGTGATTTATAGACCA
AAGTAAATTAATTAATGATCATTGTTGATTCATACCACAGGGAATGGTGCTTTTATGGCT
AACTGCA

Last Updated on 06.12.2018 16:11