Sequences from confirmation amplicon


Confirmation sequences for line 138G06 and hit At3g20510 are shown.
Line ID 138G06
Locus At3g20510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7161162
Primers used for PCR CD54 - 8474, show primer details
   
Sequence Name 09-K104268-0022-138-G06C-CD54
GenBank Accession LN490157 [GenBank]
Sequencing primer gene specific primer, CD54: 5'-GCTTTGTGTTGTTTTTCCCACTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K104268-0022-138-G06C-CD54
TGACATACGTTAGATTTTGTCATGTAGATGGTTCAACGAATGTGACATACAAAACAATGT
TGAAAACTATTTCCTTTTGAACATTACTCACATTAGGTACAACAACTGTCACTATCTTAA
AATGCAATACTAAAAAACATTCTTCATCACATTAATATAAAGTTGAGATACATATAAAAT
TACAAATTAATTACAATTAGGCCACAAAAATGATGGACCGTAAGTGGAGGATTAGTTGTC
TGAAGACCGTTGACAAAAAAAGGCGGACCTTTTCCACGCGCTCACCACACAACACTTTCA
TAAAATCAATTCATCGATCCATCGTCTCTATCAAATCAATTCATCGATTGCGATCGCTCT
TTCAATTTTCTTCATCACCGGTGAAAATAATCGAAACCCAGCACGAGAAAAATGCATGAT
TTTTGCTTCACTATCCCGTACGGGATGCTTCTCATCGGCGGAGGATTTATCGGGTACATG
AAGAAAGGAAGTATAACATCTTTCGCCGGAGGTGCAGGCACTGGATTATTACTCATTCTC
GCTGGTTATATCAGTCTCAAAGCTTTTGAGAAGAAGAAGAATTCCACTATTGCTATGGTT
CTTCAGACAGGTTTTTTTCTTGCAAATTTCTTTCCTTTTTAGCGAACTGGGTTTATATGT
CTTTTAACAAATATTTATGTTTA
   
Sequence Name 25-K104269-0022-138-G06C-8409
GenBank Accession LN497422 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K104269-0022-138-G06C-8409
TAATCTTCCTAAACATAAATATCTGTTAAAGACATATAAACCCACATCGCTAAAAAGGAA
AGAAATTTGCAAGAAAAAAACCTGTCTGAAGAACCATAGCAATAGTGGAATTCTTCTTCT
TCTCAAAAGCTTTGAGACTGATATAACCAGCGAGAATGAGTAATAATCCAGTGCCTGCAC
CTCCGGCGAAAGATGTTATACTTCCTTTCTTCATGTACCCGATAAATCCTCCGCCGATGA
GAAGCATCCCGTACGGGATAGTGAAGCAAAAATCATGCATTTTTCTCGTGCTGGGTTTCG
ATTATTTTCACCGGTGATGAAGAAAATTGAAAGAGCGATCGCAATCGATGAATTGATCTG
ATAGAGACGATGGATCGATGAATTGATTTTATGAAAGTGTTGTGTGGTGAGCGCGTGGAA
AAGGTCCGCCTTTTTTCGTCAACGGTCTTCAGACAACTAATCCTCCACTTACGGTCCATC
ATTTTTGTGGCCTAATTGTAATTAATTTGTAATTTTATATGTATCTCAACTTTATATTAA
TGTGATGAAGAATGTTTTTTAGTATTGCATTTTAAGATAGTGACAGTTGTTGTACCTAAT
GTGAGTAATGTTCAAAAGGAAATAGTTTTCAACATTGTTTTTGTATGTCACATTCGTTGA
ACCATCTACATTGAACAAATCTAACGTATGTCACTTTAGCTTCTAAATTTTTTTTTTAAG
AATATAAATAGGGGGAAAAA

Last Updated on 06.12.2018 16:11