Sequences from confirmation amplicon


Confirmation sequences for line 139A09 and hit At4g31520 are shown.
Line ID 139A09
Locus At4g31520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15278493
Primers used for PCR 08cv - 3144, show primer details
   
Sequence Name 30-K109341-0022-139-A09C-08cv
GenBank Accession LN499757 [GenBank]
Sequencing primer gene specific primer, 08cv: 5'-TCGATCAAATTTCACGGTACATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K109341-0022-139-A09C-08cv
AAGAGTTTTGCTCTCAATTTCACTTCCAAGCTTTCCTTCCTCTGAACTGGCTTCCGCTGT
GGCTATTCTTCATTTTCTTGACTCGCTTTGCTTTCCCAGCCTTTGATCTTACTGCAGCGA
GAGGCATATTCTTCTTGTGTTCCTTCTGTCTATTGCTCGATCCTCCGGTCTGCGTGTTTT
CATTTCACACAATTATTGGATCATCAAACAACACACAGCCTATATATGCTTGACACAATT
GTAAATGGCTTCGAATGAAATGTTTGGGACCGAAACGACAATCTGATCCCCGGGTACCCT
GTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGG
GATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCA
   
Sequence Name 14-K109340-0022-139-A09C-3144
GenBank Accession LN492549 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K109340-0022-139-A09C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCGGTCCCAAACATTTCATTCGAAGCCATTTAC
AATTGTGTCAAGCATATATAGGCTGTGTGTTGTTTGATGATCCAATAATTGTGTGAAATG
AAAACACGCAGACCGGAGGATCGAGCAATAGACAGAAGGAACACAAGAAGAATATGCCTC
TCGCTGCAGTAAGATCAAAGGCTGGGAAAGCAAAGCGAGTCAAGAAAATGAAGAATAGCC
ACAGCGGAAGCCAGTTCAGAGGAAGGAAAGCTTGGAAGTGAAATTGAGAGCAAAACTTCT
TTTTTATTTATTTGTTTTCTTGCATAATGTACCGTGAAATTTGATCG

Last Updated on 06.12.2018 16:11