Sequences from confirmation amplicon


Confirmation sequences for line 139C06 and hit At3g58650 are shown.
Line ID 139C06
Locus At3g58650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21698571
Primers used for PCR 09vh - 8474, show primer details
   
Sequence Name 59-K117932-0022-139-C06C-09vh
GenBank Accession LN510099 [GenBank]
Sequencing primer gene specific primer, 09vh: 5'-TCTCCCAACTCTCTGGTATGTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K117932-0022-139-C06C-09vh
TATGAAGTTTAATAATCTAAAGTTTCTTCTTTTAAACAGAAGAATCTAAGCAAGCAAAAG
AGAATGTTCAAAACCCCTCTATTACGCCGCATTCTGTTGTAAGCCTTTTCTTACTCAACA
CCATTTTCCTTGTTTGATGCAATGTTTTGTTTTGTGTTAGTTTACTGAATGACATGAACT
ATTTGTGTTCTTTGGTGTGATCTGTTTACAACAGTTTGAAGTTGATCAGAGTGTAAAGAA
CCCAACTTACAACCCGAGAAGTGACTCTAGCTGTTGTGCTTCATCTGTTACTAGTGATGA
TGGAAATGTGGTTAGAGCAAGTGTGGTAGCGAGGCTTATGGGTTTAGAGGGTTTACCACT
ACCAAACGTTTTGGAACCACGGGTTAATCCTGATCTTGATCCATACTTTCTCAGATCCTC
GCGCCAAGCAAACACTTGGGATGCTAATGTTGATCGTCAGAGTGACTTCGATGGTGTTTC
TTGGGATCATTTGGATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTACATGTCCG
   
Sequence Name 19-K117931-0022-139-C06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K117931-0022-139-C06C-8409
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGGTTA

Last Updated on 06.12.2018 16:11