Sequences from confirmation amplicon


Confirmation sequences for line 139D03 and hit At5g19670 are shown.
Line ID 139D03
Locus At5g19670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6649144
Primers used for PCR 35zg - 8474, show primer details
   
Sequence Name 21-K127062-0022-139-D03C-35zg
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 35zg: 5'-AATTCTCACAACCGCACTAACCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K127062-0022-139-D03C-35zg
TCAGTTCCAGTACCCTTTCTTCATAGAACCGATGGAGCCGATCATTTTCTTGTTGCTTGT
CATGATTGGGTAGGCATCTGTTTCATTTTTTAAAGTTAACTAATCATTTTTTCTATAGGT
AACAATTTTCTATGTTGAATTTGGCTTCACTAACAGGCACCATATGAGACTAGGCACCAC
ATGGAGCATTGCATTAAAGCTTTATGCAATGCAGACGTAACCGCAGGCTTTAAGATTGGA
AGAGACATCTCACTCCCTGAAACATATGTCAGAGCCGCCAAAAATCCGCTTCGTGATTTG
GGTGGAAAACCGCCGTCTCAGAGGCGGACCCTGGCTTTCTATGCTGGAAGCATGCACGGT
TACTTACGTCAGATTCTGCTACAGCATTGGAAAGACAAAGACCCGGACATGAAAATCTTC
GGGCGAATGCCATTTGGTGTGGCGAGCAAGATGAATTACATCGAGCAGATGAAAAGCAGC
AAGTACTGCATTTGTCCTAAAGGGTACGAAGTCAACAGCCCGAGGGTGGTTGAATCGATT
TTCTATGAGTGTGTCCCTGTGATCATCTCCGACAACTTTGTGCCACCTTTCTTCGAGGTT
CTTGATTGGAGAACAATGCAATATGGAGATATTTCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 05-K127061-0022-139-D03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K127061-0022-139-D03C-8409
CATTTACAATTGAATATATCCTGAAATATCTCCATATTGCATTGTTCTCCAATCAAGAAC
CTCGAAGAAAGGTGGCACAAAGTTGTCGGAGATGATCACAGGGACACACTCATAGAAAAT
CGATTCAACCACCCTCGGGCTGTTGACTTCGTACCCTTTAGGACAAATGCAGTACTTGCT
GCTTTTCATCTGCTCGATGTAATTCATCTTGCTCGCCACACCAAATGGCATTCGCCCGAA
GATTTTCATGTCCGGGTCTTTGTCTTTCCAATGCTGTAGCAGAATCTGACGTAAGTAACC
GTGCATGCTTCCAGCATAGAAAGCCAGGGTCCGCCTCTGAGACGGCGGTTTTCCACCCAA
ATCACGAAGCGGATTTTTGGCGGCTCTGACATATGTTTCAGGGAGTGAGATGTCTCTTCC
AATCTTAAAGCCTGCGGTTACGTCTGCATTGCATAAAGCTTTAATGCAATGCTCCATGTG
GTGCCTAGTCTCATATGGTGCCTGTTAGTGAAGCCAAATTCAACATAGAAAATTGTTACC
TATAGAAAAAATGATTAGTTAACTTTAAAAAATGAAACAGATGCCTACCCAATCATGACA
AGCAACAAGAAAATGATCGGCTCCATCGGTTCTATTGAAGAAAGGGTACTTGGAACTGAT
GTGCTCCGTATATTCCTTAAGAAACTGGCGTAGGTTAGTGCGGTGGTGAGAA

Last Updated on 06.12.2018 16:11