Sequences from confirmation amplicon


Confirmation sequences for line 140A08 and hit At2g18230 are shown.
Line ID 140A08
Locus At2g18230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7932668
Primers used for PCR CD52 - 8474, show primer details
   
Sequence Name 37-K104189-0022-140-A08C-CD52
GenBank Accession LN502598 [GenBank]
Sequencing primer gene specific primer, CD52: 5'-CGACTTTCTTGTTCTCGTTCTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K104189-0022-140-A08C-CD52
GTTGTAGTGAACTGAAACAATCATAAGCGAAAACAAAATTATACGTTAAATGCAACATGT
CTCTGTAGGATACAGTCCTCAAAGAAGCGACGGATTTCAGCTAGACGGTGAGGGGGAAGC
TCTTTGATGTCTCTGTAGTGACGGAACTCGGGATCATCAGCACATACTGCAATGATCTTG
TCGTCTTTCTCACCCTGCAAGTTTCAGAACAAGGTGATTCCTGGTTTCTTACTAAGGTAA
TACATTACATAGAGGTAGAATTGGGTTTAAGAGTTTTTGTTACCTGATCAATCATGGGCA
TTAGACAATTACCAAACCTTATTCAATTTATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 53-K104190-0022-140-A08C-8409
GenBank Accession LN508140 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K104190-0022-140-A08C-8409
TTCGGACTGAGCCATTTACATTGAATAAATTGAATAAGGTTTGGTAATTGTCTAATGCCC
ATGATTGATCAGGTAACAAAAACTCTTAAACCCAATTCTACCTCTATGTAATGTATTACC
TTAGTAAGAAACCAGGAATCACCTTGTTCTGAAACTTGCAGGGTGAGAAAGACGACAAGA
TCATTGCAGTATGTGCTGATGATCCCGAGTTCCGTCACTACAGAGACATCAAAGAGCTTC
CCCCTCACCGTCTAGCTGAAATCCGTCGCTTCTTTGAGGACTGTATCCTACAGAGACATG
TTGCATTTAACGTATAATTTTGTTTTCGCTTATGATTTGTTTTCAGTTTTCACTTAACAA
ACAATGTTTTTAGACAAGAAGAACGAGAACAAGAAAGTCG
Primers used for PCR CD53 - 3144, show primer details
   
Sequence Name 44-K104270-0022-140-A08C-CD53
GenBank Accession LN505172 [GenBank]
Sequencing primer gene specific primer, CD53: 5'-ACCGTAAGAGTCGAAATGAAGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K104270-0022-140-A08C-CD53
TCGCGTTCTCTACTCATCCATTGTGTACCCCCACAACTACGGTTTCATCCCTCGAACTAT
CTGTGAAGACAGTGATCCAATGGATGTCCTGGTACTGATGCAGGTGCTTTCTCTCGCCTC
ATCTTAGCTTTCATGTTCATACAAACTTGATGTAAACAGGCAGTGAAATAACAGACTTGA
GATAAAATACTGTGCAGGAGCCTGTGCTAACCGGATCATTCCTCCGTGCCCTGATAGTTT
GCGGGTACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCC
TGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTG
GGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCA
   
Sequence Name 60-K104271-0022-140-A08C-3144
GenBank Accession LN510250 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K104271-0022-140-A08C-3144
TCCACTATCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTACCCGC
AAACTATCAGGGCACGGAGGAATGATCCGGTTAGCACAGGCTCCTGCACAGTATTTTATC
TCAAGTCTGTTATTTCACTGCCTGTTTACATCAAGTTTGTATGAACATGAAAGCTAAGAT
GAGGCGAGAGAAAGCACCTGCATCAGTACCAGGACATCCATTGGATCACTGTCTTCACAG
ATAGTTCGAGGGATGAAACCGTAGTTGTGGGGGTACACAATGGATGAGTAGAGAACGCGA
TCAACCTAGAGAGGAAAACTTTCAGTGATCAGAGTTTTTCTTCATTTCGAC

Last Updated on 06.12.2018 16:11