Sequences from confirmation amplicon


Confirmation sequences for line 140E03 and hit At2g32020 are shown.
Line ID 140E03
Locus At2g32020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13631644
Primers used for PCR 02vz - 8474, show primer details
   
Sequence Name 54-K118278-0022-140-E03C-02vz
GenBank Accession LN508621 [GenBank]
Sequencing primer gene specific primer, 02vz: 5'-TCCCTCTAACCTCACTTCTGTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K118278-0022-140-E03C-02vz
TAGGATTTATAGATTTTTTTGGCAATGAAAAACAGAGTGTTTACATCATGTATTACGTAT
ATGAATCATACACTAAATCCAACATATATATGTAGAATTGCTAATTAATCTTTTTGTTGA
TTAATTACTTCAAAGTATCAGTAGACAAGAAACTAAACATAACAGTGTCTCTTACACTTC
CCTTGATACATATAAACTTCCTCATCACACCTTCTCTAGTAAACCCAACTTTTTCAAGAA
CCCTTTGAGATCCAACATTGTCCACATCAACAAGTGCCTCAAGCCTTTCAATCTCTGGAA
ATTCCTCGAATACCTCCGCGGTCACTAGCCTCACTGCTTCCGTGGCAAACCCTTTTCCCC
AATACTTTCTCGCTAGGACATAACCAATCTCTTTTCTGATGTTATCAACCGCCATGATCA
AGATGTAGCCGATTGGACGGTCGTCTTCTAAGCAGATGGCTCGGAGCCAAGGGTGTGTCA
GGACACGATCGGTTATGTATTTGATAGCTTCGTCTCGGCTCGTGCAAGGCTCCCAAGTGC
AAAATCGAGCCACTTTAGGATCTGTGGCCCAAACCATGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAG
   
Sequence Name 22-K118277-0022-140-E03C-8409
GenBank Accession LN496307 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K118277-0022-140-E03C-8409
CATTTACTGGTTTGGGCCACAGATCCTAAAGTGGCTCGATTTTGCACTTGGGAGCCTTGC
ACGAGCCGAGACGAAGCTATCAAATACATAACCGATCGTGTCCTGACACACCCTTGGCTC
CGAGCCATCTGCTTAGAAGACGACCGTCCAATCGGCTACATCTTGATCATGGCGGTTGAT
AACATCAGAAAAGAGATTGGTTATGTCCTAGCGAGAAAGTATTGGGGAAAAGGGTTTGCC
ACGGAAGCAGTGAGGCTAGTGACCGCGGAGGTATTCGAGGAATTTCCAGAGATTGAAAGG
CTTGAGGCACTTGTTGATGTGGACAATGTTGGATCTCAAAGGGTTCTTGAAAAAGTTGGG
TTTACTAGAGAAGGTGTGATGAGGAAGTTTATATGTATCAAGGGAAGTGTAAGAGACACT
GTTATGTTTAGTTTCTTGTCTACTGATACTTTGAAGTAATTAATCAACAAAAAGATTAAT
TAGCAATTCTACATATATATGTTGGATTTAGTGTATGATTCATATACGTAATACATGATG
TAAACACTCTGTTTTTCATTTGCCAAAAAAATCTATAAATCCTACATTGTAAATTACGAA
ACAATTAAAGTCAGTGCATGAGACAGAAGTG

Last Updated on 06.12.2018 16:11