Sequences from confirmation amplicon


Confirmation sequences for line 140F05 and hit At1g55970 are shown.
Line ID 140F05
Locus At1g55970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20938099
Primers used for PCR IL20 - 8474, show primer details
   
Sequence Name 28-K032415-022-140-F05C-8409
GenBank Accession LN498729 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K032415-022-140-F05C-8409
CGGACTGAGCCTTTACAATTGAATAAGAGAGAGCGAAGATGATTATGTTACATTCTATTT
CTTAAAGCAGTGCGTCTCTTCTTTATGAAATTATTAGCATCCTGCTGAGGAGAAATAACA
GTTATTGGTGCCGACGATGACGAAGAAACAGCAACAGAGTTCTGAGGAACTTCTTTATTA
TTATTCATATTTTTCTTTCAACAGAGCATGTGACCAAAGCCTCCTAGCTCCTCCTATTTT
TGACCTGAAGAAAAAGTAATTGATTTTAGGGCTCCGCCTAAGTCAAAAAACAAAGGTCAA
TTATATAAGGTTCGCAATTTGATTAATTAGCGTTGAGAAGCCAAATACAGTCAACGAGAA
CGAAGACGACCAAAGAAACAAAGGATGGTCTTGGTCGATGAGTTAAGATACTACACTACG
TGCGATTGTGACGGGGAACTATGGGACGCATGAAGAGGAGGAATAGGGATTTGTCTTTTA
TATCCAAAAAATCAATCAAGCCCTTTATTTTATTTATTTATTTATTTAGTTGACAAAAGT
TATATAAATCAACTAGTTTGACTTTTGTTTTTGACGGAT
   
Sequence Name 28-K032415-022-140-F05C-IL20
GenBank Accession LN498730 [GenBank]
Sequencing primer gene specific primer, IL20: 5'-ACTTTCCTAGTTGATTCCGTCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K032415-022-140-F05C-IL20
ACTTTTGTCACTAAATAAATAAATAAATAAAATAAAGGGCTTGATTGATTTTTTGGATAT
AAAAGACAAATCCCTATTCCTCCTCTTCATGCGTCCCATAGTTCCCCGTCACAATCGCAC
GTAGTGTAGTATCTTAACTCATCGACCAAGACCATCCTTTGTTTCTTTGGTCGTCTTCGT
TCTCGTTGACTGTATTTGGCTTCTCAACGCTAATTAATCAAATTGCGAACCTTATATAAT
TGACCTTTGTTTTTTGACTTAGGCGGAGCCCTAAAATCAATTACTTTTTCTTCAGGTCAA
AAATAGGAGGAGCTAGGAGGCTTTGGTCACATGCTCTGTTGAAAGAAAAATATGAATAAT
AATAAAGAAGTTCCTCAGAACTCTGTTGCTGTTTCTTCGTCATCGTCGGCACCAATAACT
GTTATTTCTCCTCAGCAGGATGCTAATAATTTCATAAAGAAGAGACGCACTGCTTTAAGA
AATAGAATGTAACATAATCATCTTCGCTCTCTCTTTATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGA
AAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAA
ACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACTTCCCCCAAAAAG
Primers used for PCR FQ14 - 8474, show primer details
   
Sequence Name 49-K035338-022-140-F05C-8409
GenBank Accession LN506752 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K035338-022-140-F05C-8409
CCATTTATCATGATCTTATCTTTAATTTGCTATATTAACCATCATATATTCTTTCTCCAG
TTATGCTATCGTAAGACACAAGCAGAAGCAGCATCAAATCTTTCTAGACAAGAAGAATCA
GCAGCAACAGCAGCGTGTTGATGATGCCACGCAACGTGCTCTCTTAGAGAAGCAGGATCA
ACAGTGTATTGCGGCAACAAGAATGATTGAGGAAGAACTCCTCAAGTCTTCTCGATCATT
TGAGGAGTACTTCGATTTAAGAACTTTTGACGCCCGTGTACGAACTATATTGCAACAGCT
TGGAACAATGCTTTCTCAGCGGCGTGCTGCTGCTGCTGCCATGAATAACGGAGAGGCTCA
ATGTATTACATCTACCCGAGCGGTCCATACTTCCATAAGCGTTAGTAACTCTTTCCAATG
CGGAAGAAGCCTTGTGCCAATTAATTGCACCACTGCAACAGCTGGAGGTACACATCTTTG
ATCTCTCCTGATTCTTTTTCAAATTATACTGACATGCTGTTCTTTATTTTGACTCTATAC
CTTATCATCT
   
Sequence Name 49-K035338-022-140-F05C-FQ14
GenBank Accession LN506753 [GenBank]
Sequencing primer gene specific primer, FQ14: 5'-AGAAAATGCTGCAGATGATAAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K035338-022-140-F05C-FQ14
GCATGTCAGTATAATTTGAAAAAGAATCAGGAGAGATCAAAGATGTGTACCTCCAGCTGT
TGCAGTGGTGCAATTAATTGGCACAAGGCTTCTTCCGCATTGGAAAGAGTTACTAACGCT
TATGGAAGTATGGACCGCTCGGGTAGATGTAATACATTGAGCCTCTCCGTTATTCATGGC
AGCAGCAGCAGCACGCCGCTGAGAAAGCATTGTTCCAAGCTGTTGCAATATAGTTCGTAC
ACGGGCGTCAAAAGTTCTTAAATCGAAGTACTCCTCAAATGATCGAGAAGACTTGAGGAG
TTCTTCCTCAATCATTCTTGTTGCCGCAATACACTGTTGATCCTGCTTCTCTAAGAGAGC
ACGTTGCGTGGCATCATCAACACGCTGCTGTTGCTGCTGATTCTTCTTGTCTAGAAAGAT
TTGATGCTGCTTCTGCTTGTGTCTTACGATAGCATAACTGGAGAAAGAATATATGATGGT
TAATATAGCAAATTAAAGATAAGATCATGATAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA

Last Updated on 06.12.2018 16:11